Tessie's sequential extraction method was used to analyze different forms of silicon (IEF-Si, CF-Si, IMOF-Si and OSF-Si) in surface sediments from a subtropical mountain deepwater reservoir-Shame Reservoir in Quanzhou City, and their temporal and spatial distribution were investigated. Total concentrations of silicon in surface sediments ranged in 3043.50~4414.24, 2711.17~3676.89, 3198.44~4444.28 mg/kg in normal water period, dry season and wet season, respectively. Total concentrations of silicon in surface sediments of this reservoir from high to low was as follows: tail zone of reservoir, central zone of reservoir, near-dam zone, storage zone. IEF-Si and CF-Si accounted for a small proportion of total silicon, but they were active species. Although IEF-Si content was low, its potential release was likely to impact the trophic state of aquatic ecosystem and the primary production. CF-Si was sensitive to environmental factors, especially pH. The percentages of IMOF-Si and OSF-Si were much larger than those of other two forms. Mobility of IMOF-Si was probably the main way to supply Si in the Shanmei Reservoir. OSF-Si was relatively stable and its distribution was linked with the sediment type and environmental. The concentrations of CF-Si and IMOF-Si were positively correlated during normal water period (P ©, 2015, Chinese Society for Environmental Sciences. All right reserved.