Antibacterial activity of carbon-coated zinc oxide particles

被引:0
作者
Sawai, Jun [1 ]
Yamamoto, Osamu [2 ]
Özkal, Burak [3 ]
Nakagawa, Zenbe-E. [2 ]
机构
[1] Department of Applied Bioscience, Faculty of Engineering, Kanagawa Institute of Technology, Atsugi, Kanagawa 243-0292
[2] Center for Geo-Environmental Science, Faculty of Engineering and Resource Science, Akita University, Akita 010-8502
[3] Metallurgical and Materials Engineering, Chemical and Metallurgical Faculty, Istanbul Technical University
关键词
Antibacterial activity; Carbon-coating; Conductimetric assay; Poly(vinyl alcohol); Zinc oxide;
D O I
10.4265/bio.12.15
中图分类号
学科分类号
摘要
Particles of ZnO coated with carbon (ZnOCC) were prepared and evaluated for their antibacterial activity. ZnO powder and poly (vinyl alcohol) (PVA) (polymerization degree: 2,000-95,000) were mixed at a mass ratio (ZnO/PVA) of 1, and then heated at 500-650°C for 3 h under argon gas with a flow rate of 50ml/min. Carbon deposited on the ZnOCC surface was amorphous as revealed by X-ray diffraction studies. The ZnOCC particles maintained their shape in water, even under agitation. The antibacterial activity of ZnOCC powder against Staphylococcus aureus was evaluated quantitatively by measuring the change in the electrical conductivity of the growth medium caused by bacterial metabolism (conductimetric assay). The conductivity curves obtained were analyzed using the growth inhibition kinetic model proposed by Takahashi for calorimetric evaluation, allowing the estimation of the antibacterial efficacy and kinetic parameters of ZnOCC. In a previous study, when ZnO was immobilized on materials, such as activated carbon, the amount of ZnO immobilized was approximately 10-50%, and the antibacterial activity markedly decreased compared to that of the original ZnO. On the other hand, the ZnOCC particles prepared in this study contained approximately 95% ZnO and possessed antibacterial activity similar to that of pure ZnO. The carbon-coating treatment could maintain the antibacterial efficacy of the ZnO and may be useful in the development of multifunctional antimicrobial materials.
引用
收藏
页码:15 / 20
页数:5
相关论文
共 28 条
  • [1] Akiyama H., Yamazaki O., Kanzaki H., Tada J., Arata J., Effect of zinc oxide on the attachment of Staphylococcus aureus, J. Dermatol. Sci, 17, pp. 67-74, (1998)
  • [2] Akiyama H., Yamasaki O., Tada J., Arata J., Calcium oxide and magnesium oxide inhibit plasma coagulation by Staphylococcus aureus cells at the lower concentration than zinc oxide, J. Dermatol. Sci, 22, pp. 62-65, (1999)
  • [3] Allen I., Newman H., Wilson M., Antibacterial activity of particulate bioglass against supra-and subgingival bacteria, Biomaterials, 22, pp. 1683-1687, (2001)
  • [4] Bari M.L., Kusunoki H., Furukawa H., Ikeda H., Isshiki K., Uemura T., Inhibition of growth of Escherichia coli O157:H7 in fresh radish (Raphanus sativus L.) sprout production by calcinated calcium, J. Food Prot, 62, pp. 128-132, (1999)
  • [5] Bari M.L., Inatsu Y., Kawasaki S., Nazuka E., Isshiki K., Calcinated calcium killing of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on the surface of tomatoes, J. Food Prot, 65, pp. 1706-1711, (2002)
  • [6] Eden G., Eden R., Enumeration of microorganisms by their dynamics ac conductance patters, IEEE Trans. Biomed. Eng, 31, pp. 193-198, (1984)
  • [7] Inagaki M., Hirose Y., Matsunaga T., Tsumura T., Toyoda M., Carbon coating of anatase-type TiO<sub>2</sub> through their precipitation in PVA aqueous solution, Carbon, 41, pp. 2619-2624, (2003)
  • [8] Kourai H., Immobilized microbiocide (in Japanese), J. Antibact. Antifung. Agents, 21, pp. 331-337, (1993)
  • [9] Lanzanova M., Mucchetti G., Neviani E., Analysis of conductance changes as a growth index of lactic acid bacteria in milk, J. Dairy Sci, 70, pp. 20-28, (1993)
  • [10] Megha G., Matthews K.R., Efficacy of chlorine and calcinated calcium treatment of alfalfa seeds and sprouts to eliminate Salmonella, Int. J. Food Microbiol, 87, pp. 301-306, (2003)