Nano-chemical infrared imaging of membrane proteins in lipid bilayers

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[1] [1,Berweger, Samuel
[2] Nguyen, Duc M.
[3] 1,Muller, Eric A.
[4] Bechtel, Hans A.
[5] 3,Perkins, Thomas T.
[6] 1,Raschke, Markus B.
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Raschke, M.B. (markus.raschke@colorado.edu) | 1600年 / American Chemical Society卷 / 135期
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The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone; we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy; with potential extension to a wide range of biomolecular systems. © 2013 American Chemical Society;
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