Nano-chemical infrared imaging of membrane proteins in lipid bilayers

被引：0
|
作者：
机构：
[1] [1,Berweger, Samuel
[2] Nguyen, Duc M.
[3] 1,Muller, Eric A.
[4] Bechtel, Hans A.
[5] 3,Perkins, Thomas T.
[6] 1,Raschke, Markus B.
来源：
Raschke, M.B. (markus.raschke@colorado.edu) | 1600年 / American Chemical Society卷 / 135期
关键词：
The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone; we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy; with potential extension to a wide range of biomolecular systems. © 2013 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
相关论文
共 50 条
  • [1] Nano-Chemical Infrared Imaging of Membrane Proteins in Lipid Bilayers
    Berweger, Samuel
    Nguyen, Duc M.
    Muller, Eric A.
    Bechtel, Hans A.
    Perkins, Thomas T.
    Raschke, Markus B.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (49) : 18292 - 18295
  • [2] Infrared spectroscopy of proteins and peptides in lipid bilayers
    Tamm, LK
    Tatulian, SA
    QUARTERLY REVIEWS OF BIOPHYSICS, 1997, 30 (04) : 365 - 429
  • [3] Mobilizing and imaging membrane-associated proteins in supported lipid bilayers.
    Boxer, SG
    Kam, L
    Ajo, C
    Ishii, C
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 223 : U458 - U458
  • [4] Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers
    Seantier, Bastien
    Dezi, Manuela
    Gubellini, Francesca
    Berquand, Alexandre
    Godefroy, Cedric
    Dosset, Patrice
    Levy, Daniel
    Milhiet, Pierre-Emmanuel
    JOURNAL OF MOLECULAR RECOGNITION, 2011, 24 (03) : 461 - 466
  • [5] Solution NMR studies on membrane proteins in lipid bilayers
    Ansorge, Philipp
    Zerbe, Oliver
    PROTEIN SCIENCE, 2016, 25 : 113 - 114
  • [6] Organization of membrane-associated proteins in lipid bilayers
    Liang, Q.
    Ma, Y. -Q
    EUROPEAN PHYSICAL JOURNAL E, 2008, 25 (02): : 129 - 138
  • [7] Lipid bilayers: an essential environment for the understanding of membrane proteins
    Page, Richard C.
    Li, Conggang
    Hu, Jian
    Gao, Fei Philip
    Cross, Timothy A.
    MAGNETIC RESONANCE IN CHEMISTRY, 2007, 45 (SUPPL.) : S2 - S11
  • [8] Lipid bilayers on polyacrylamide brushes for inclusion of membrane proteins
    Smith, EA
    Coym, JW
    Cowell, SM
    Tokimoto, T
    Hruby, VJ
    Yamamura, HI
    Wirth, MJ
    LANGMUIR, 2005, 21 (21) : 9644 - 9650
  • [9] The Effect of Lipid Bilayers on Membrane-Bound Proteins
    Sapp, Kayla
    Maibaum, Lutz
    BIOPHYSICAL JOURNAL, 2015, 108 (02) : 77A - 77A
  • [10] Quantifying the Diffusion of Membrane Proteins and Peptides in Lipid Bilayers
    Weiss, Kerstin
    Enderlein, Joerg
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 87A - 87A
12345