Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by Coconut Water

被引：30

作者：

Al-Khayri J.M. ^{[1
]}

机构：

[1] Department of Agricultural Biotechnology, College of Agricultural and Food Sciences, King Faisal University, Al-Hassa 31982

来源：

Biotechnology | 2010年 / 9卷 / 04期

关键词：

Coconut Water; Complex organic additives; Date palm; In vitro; Micropropagation; Somatic embryogenesis;

D O I：

10.3923/biotech.2010.477.484

中图分类号：

学科分类号：

摘要：

Micropropagation systems often require optimization for different genotypes by modifying culture medium. Improved regeneration in numerous plant species was attained using Coconut Water (CW) as a medium additive. The objective of the study was to evaluate callus growth and somatic embryogenesis of two commercial cultivars of date palm (Phoenix dactylifera L.) in response to CW concentrations and sterilization methods, coautoclaving or microfiltration. Calli induced from shoot tip explants, were grown on multiplication medium supplemented with CW at 0 to 20% (v/v). To induce somatic embryogenesis, callus was transferred to a hormone-free regeneration medium containing the corresponding CW concentrations. Results have shown improved callus growth and enhanced somatic embryogenesis in response to CW. Callus fresh weight and numbers of resultant embryos were directly proportional to CW concentration reaching optimum levels at 10 and 15% CW for cv. Khasab and cv. Nabout Saif, respectively. Both coautoclaving and microfiltering CW were equally effective in promoting culture growth and differentiation; however, coautoclaved CW generally gave increased responses. Furthermore, CW enrichment expedited the induction somatic embryos and increased germination percentage. Potentially this may contribute to reducing the cost of commercial micropropagation of date palm. © 2010 Asian Network for Scientific Information.

引用

页码：477 / 484

页数：7

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