Preparation and characterization of polyvinyl alcohol and chitosan composite film with cassia oil encapsulated in β-cyclodextrin and its application in fresh banana

In this study, composite films were developed by encapsulating cassia oil (CO) with beta-cyclodextrin through a microencapsulation technique and incorporating it into a chitosan (CS), polyvinyl alcohol (PVA) and glycerol matrix. The primary objective of the film was to inhibit bacterial growth on the surface of fresh bananas and extend their shelf life. Characterization methods were employed to evaluate the physical properties and functionality of the composite films. FTIR, XRD, and SEM analyses demonstrated that cassia oil microcapsules (COM) were uniformly dispersed throughout the film and exhibited excellent compatibility with the matrix. The inclusion of 30 % COM improved the film's UV-blocking properties from 86.15 % to 91.03 %. Additionally, due to its hydrophobic nature, CO significantly reduced the water content to 9.02 % and 10.67 %. Furthermore, the COM enhanced the film's tensile strength from 21.18 MPa to 43.21 MPa, and increased its antioxidant capacity to 36.87 %. The results also indicated that 30 % COM significantly enhanced the film's antimicrobial activity against Escherichia coli and Staphylococcus aureus with inhibition zone diameters of 12.5 mm and 11.5 mm, while maintaining biosafety, as evidenced by unaltered cell survival rates in BEAS-2B and L02 cells. The film containing 30 % COM exhibited excellent preservation capacity for bananas, effectively extending their shelf life. These findings suggest that films containing COM have the potential to replace traditional plastic packaging in practical applications.