Development and Validation of RP-HPLC Method for the Estimation of Lisinopril in Tablet Dosage Form

被引：0
作者：
Chander, Vikas [1 ]
Mohan, Manindra [2 ]
Seth, Richa [2 ]
Singh, Prashant [2 ]
Singh, Rakesh [3 ]
Gupta, Sanjay [4 ]
机构：
[1] Himalayan Institute of Pharmacy and Research, Uttarakhand, Dehradun
[2] Department of Chemistry, DAV (PG) College, Uttarakhand
[3] Department of Chemistry, DBS (PG) College, Uttarakhand
[4] Department of Biotechnology and Biochemistry, SBS (PG) Institute of Biomedical Sciences & Research, Balawala, Uttarakhand, Dehradun
来源：
Analytical Chemistry Letters | 2012年 / 2卷 / 05期
关键词：
Lisinopril; method validation; nucleosil; RP-HPLC; tablet dosage form;
D O I：
10.1080/22297928.2012.10648282
中图分类号：
学科分类号：
摘要：
A new isocratic reversed-phase high performance liquid chromatographic (HPLC) method with diode-array UV detection was developed and validated for the determination of lisinopril in pharmaceutical formulation. The method validation of lisinopril was performed by using Nucleosil (125 x 4.0mm, 5mm) as stationary phase with mobile phase consisting of buffer solution, isopropyl alcohol and triethylamine (95:5:0.1) at a flow rate of 1.0 ml/min. The column temperature and spectrometric detection were monitored at 50°C and 215 nm, respectively. The 20 μl of sample was injected for the run time of 7 min. The statistical analysis of data showed that the validated method is within limits in all respective parameters and is quick and reliable for quantitative as well as quality control analysis of lisinopril for pharmaceutical formulation. © 2012, Copyright Har Krishan Bhalla & Sons.
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页码：309 / 313
页数：4
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