In this review we discuss recent advances in the field of NMR structural studies of proteins. We mention the development of new spectrometer hardware, novel experimental techniques and sample preparation. The approaches improving obtained structures, like analysis of Residual Dipolar Couplings, and enabling studies of large molecules as for example TROSY and CRINEPT are compared. As the most important, achievements in the field of fast multidimensional NMR are discussed in details.
机构:
Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, England
机构:
Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Hongo, Tokyo 1130033, JapanUniv Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Hongo, Tokyo 1130033, Japan
Toyama, Yuki
Shimada, Ichio
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Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Hongo, Tokyo 1130033, JapanUniv Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Hongo, Tokyo 1130033, Japan