Numerical detection simulation of microchip electrophoresis in a pure pressure-driven laminar flow

This study presents a numerical detection simulation of microchip electrophoresis in a straight microchannel through the pressure-driven laminar flow. The simulation is used to calculate charged particle movement caused by the transport mechanism of convection, diffusion, and electrophoretic migration for imitating the electrophoresis detection apparatus to obtain the electropherogram. The simulated detection signals from four detectors at different positions in the microchannel are used to study the effect of time. The simulation results indicate that the effects of diffusion and the velocity profile caused by pressure gradient reduce the amplitude of detection signals with time and form the different distribution of particle patterns. The magnitude of detection area also effects the strength of detection signals.