Strategies for systems-level metabolic engineering

被引：37

作者：

Department of Chemical and Biomolecular Engineering , Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, 305-701 Daejeon, Korea, Republic of ^{[1
]}

不详 ^{[2
]}

不详 ^{[3
]}

机构：

[1] Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 305-701 Daejeon

[2] Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon

[3] Department of Bio and Brain Engineering and Bioinformatics Research Center, Korea Advanced Institute and Technology, Daejeon

来源：

Biotechnol. J. | 2008年 / 5卷 / 612-623期

关键词：

Genome; Metabolic engineering; System biology;

D O I：

10.1002/biot.200700240

中图分类号：

学科分类号：

摘要：

Bio-based production of chemicals, fuels and materials is becoming more and more important due to the increasing environmental problems and sharply increasing oil price. To make these bio-based processes economically competitive, the biotechnology industry explores new ways to improve the performance of microbial strains in fermentation processes. In contrast to the random mutagenesis and/or intuitive local metabolic engineering practiced in the past, we are now moving towards global-scale metabolic engineering, aided by various experimental and computational tools. This has recently led to some remarkable achievements for the overproduction of value-added products. In this review, we highlight several relevant gene manipulation tools and computational tools using genome-scale stoichiometric models, and provide useful strategies for successful metabolic engineering along with selected exemplary studies. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：612 / 623

页数：11

共 52 条

[1]

Henkel J., Maurer S.M., The economics of synthetic biology, Mol. Syst. Biol, 3, (2007)

[2]

Song H., Lee S.Y., Production of succinic acid by bacterial fermentation, Enzyme Microbial Technol, 39, pp. 352-361, (2006)

[3]

Otero J.M., Panagiotou G., Olsson L., Fueling industrial biotechnology growth with bioethanol, Adv. Biochem. Eng. Biotechnol, 108, pp. 1-40, (2007)

[4]

Lee S.Y., Bacterial polyhydroxyalkanoates, Biotechnol. Bioeng, 49, pp. 1-14, (1996)

[5]

Kim P.J., Lee D.Y., Kim T.Y., Lee K.H., Et al., Metabolite essentiality elucidates robustness of Escherichia coli metabolism, Proc. Natl. Acad. Sci. USA, 104, pp. 13638-13642, (2007)

[6]

Barrett C.L., Kim T.Y., Kim H.U., Palsson B.O., Lee S.Y., Systems biology as a foundation for genome-scale synthetic biology, Curr. Opin. Biotechnol, 17, pp. 488-492, (2006)

[7]

Joyce A.R., Palsson B.O., The model organism as a system: Integrating 'omics' data sets, Nat. Rev. Mol. Cell Biol, 7, pp. 198-210, (2006)

[8]

Park J.H., Lee K.H., Kim T.Y., Lee S.Y., Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knock-out simulation, Proc. Natl. Acad. Sci. USA, 104, pp. 7797-7802, (2007)

[9]

Bernal A., Ear U., Kyrpides N., Genomes OnLine Database (GOLD): A monitor of genome projects worldwide, Nucleic Acids Res, 29, pp. 126-127, (2001)

[10]

Kanehisa M., Goto S., Hattori M., Aoki-Kinoshita K.F., Et al., From genomics to chemical genomics: New developments in KEGG, Nucleic Acids Res, 34, (2006)

← 1 2 3 4 5 6 →