Strategies for systems-level metabolic engineering

被引:37
作者
Department of Chemical and Biomolecular Engineering , Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, 305-701 Daejeon, Korea, Republic of [1 ]
不详 [2 ]
不详 [3 ]
机构
[1] Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 305-701 Daejeon
[2] Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon
[3] Department of Bio and Brain Engineering and Bioinformatics Research Center, Korea Advanced Institute and Technology, Daejeon
来源
Biotechnol. J. | 2008年 / 5卷 / 612-623期
关键词
Genome; Metabolic engineering; System biology;
D O I
10.1002/biot.200700240
中图分类号
学科分类号
摘要
Bio-based production of chemicals, fuels and materials is becoming more and more important due to the increasing environmental problems and sharply increasing oil price. To make these bio-based processes economically competitive, the biotechnology industry explores new ways to improve the performance of microbial strains in fermentation processes. In contrast to the random mutagenesis and/or intuitive local metabolic engineering practiced in the past, we are now moving towards global-scale metabolic engineering, aided by various experimental and computational tools. This has recently led to some remarkable achievements for the overproduction of value-added products. In this review, we highlight several relevant gene manipulation tools and computational tools using genome-scale stoichiometric models, and provide useful strategies for successful metabolic engineering along with selected exemplary studies. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:612 / 623
页数:11
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