Probing methyl dynamics from 13C autocorrelated and cross-correlated relaxation

被引：0
|
作者：
Zhang, Xu [1 ,2 ]
Sui, Xiaogang [3 ]
Yang, Daiwen [1 ]
机构：
[1] Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
[2] State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China
[3] Department of Chemistry, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
来源：
Journal of the American Chemical Society | 2006年 / 128卷 / 15期
关键词：
An understanding of side-chain motions in protein is of great interest since side chains often play an important role in protein folding and intermolecular interactions. A novel method for measuring dipole-dipole cross-correlated relaxation in methyl groups of uniformly 13C-labeled proteins without deuteration has been developed by our group. The excellent agreement between dynamic parameters of methyl groups in ubiquitin obtained from the cross-correlated relaxation and 13C spin-lattice relaxation and those derived previously from 2H relaxation data demonstrates the reliability of the method. This method was applied to the study of side-chain dynamics of human intestinal fatty acid binding protein (IFABP) with and without its ligand. Binding of oleic acid to the protein results in decreased mobility of most of the methyl groups in the binding region; whereas no significant change in mobility was observed for methyl groups in the nonbinding region. © 2006 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：5073 / 5081
相关论文
共 50 条
  • [1] Probing methyl dynamics from 13C autocorrelated and cross-correlated relaxation
    Zhang, X
    Sui, XG
    Yang, DW
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (15) : 5073 - 5081
  • [2] Estimates of methyl 13C and 1H CSA values (Δσ) in proteins from cross-correlated spin relaxation
    Tugarinov, V
    Scheurer, C
    Brüschweiler, R
    Kay, LE
    JOURNAL OF BIOMOLECULAR NMR, 2004, 30 (04) : 397 - 406
  • [3] Estimates of methyl 13C and 1H CSA values (Δσ) in proteins from cross-correlated spin relaxation
    Vitali Tugarinov
    Christoph Scheurer
    Rafael Brüschweiler
    Lewis E. Kay
    Journal of Biomolecular NMR, 2004, 30 : 397 - 406
  • [4] Probing Methyl Group Dynamics in Proteins by NMR Cross-Correlated Dipolar Relaxation and Molecular Dynamics Simulations
    Ali, Ahmed A. A., I
    Hoffmann, Falk
    Schaefer, Lars, V
    Mulder, Frans A. A.
    JOURNAL OF CHEMICAL THEORY AND COMPUTATION, 2022, 18 (12) : 7722 - 7732
  • [5] Inversion of Hyperpolarized 13C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments
    Negroni, Mattia
    Guarin, David
    Che, Kateryna
    Epasto, Ludovica M.
    Turhan, Ertan
    Selimovic, Albina
    Kozak, Fanny
    Cousin, Samuel
    Abergel, Daniel
    Bodenhausen, Geoffrey
    Kurzbach, Dennis
    JOURNAL OF PHYSICAL CHEMISTRY B, 2022, 126 (24): : 4599 - 4610
  • [6] Studies of proline conformational dynamics in IDPs by 13C-detected cross-correlated NMR relaxation
    Schiavina, Marco
    Konrat, Ruth
    Ceccolini, Irene
    Mateos, Borja
    Konrat, Robert
    Felli, Isabella C.
    Pierattelli, Roberta
    JOURNAL OF MAGNETIC RESONANCE, 2023, 354
  • [7] New probes of ligand flexibility in drug design:: Transferred 13C CSA-dipolar cross-correlated relaxation at natural abundance
    Peng, JW
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (36) : 11116 - 11130
  • [8] Protein backbone dynamics through 13C′-13C α cross-relaxation in NMR spectroscopy
    Ferrage, Fabien
    Pelupessy, Philippe
    Cowburn, David
    Bodenhausen, Geoffrey
    Journal of the American Chemical Society, 2006, 128 (34): : 11072 - 11078
  • [9] Protein backbone dynamics through 13C′-13Cα cross-relaxation in NMR spectroscopy
    Ferrage, Fabien
    Pelupessy, Philippe
    Cowburn, David
    Bodenhausen, Geoffrey
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (34) : 11072 - 11078
  • [10] New probes of ligand flexibility in drug design: Transferred 13C CSA-dipolar cross-correlated relaxation at natural abundance
    Peng, J.W. (Jeffrey.W.Peng.6@nd.edu), 1600, American Chemical Society (125):
12345