Development of an external standard method for the high-throughput determination of broadly polar multiclass synthetic dyes in high-sugar, high-fat, and high-protein foods

被引：0

作者：

Zhang, Ting ^{[1
]}

Cheng, Jie ^{[1
]}

Huang, Xiansheng ^{[3
]}

Liang, Yuxuan ^{[5
,6
]}

Yao, Xurui ^{[4
]}

Chen, Shuixia ^{[2
]}

Wei, Xiaoqun ^{[1
,2
]}

Lei, Hongtao ^{[1
]}

机构：

[1] South China Agr Univ, Coll Food Sci, Natl Local Joint Engn Res Ctr Precis Machining & S, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

[2] Jieyang Ctr, Guangdong Prov Lab Chem & Fine Chem Engn, Jieyang 515200, Peoples R China

[3] Guangdong Mingchentang Hlth Ind Co Ltd, Jieyang 522095, Peoples R China

[4] Jieyang Market Supervis Adm, Jieyang 522095, Peoples R China

[5] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen Key Lab Food Nutr & Hlth, Shenzhen 518060, Peoples R China

[6] Shenzhen Univ, Inst Innovat Dev Food Ind, Shenzhen 518060, Peoples R China

来源：

FOOD CHEMISTRY | 2025年 / 464卷

基金：

芬兰科学院;

关键词：

Synthetic dyes quantification; Multiclass dye extraction; Polar compound separation; Food matrix interference;

D O I：

10.1016/j.foodchem.2024.141820

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Simultaneous detection of multiple synthetic dyes in food additives presents a challenge. A high-throughput UPLC-Q-OMS method with purification-free extraction was developed for the simultaneous detection of 30 synthetic dyes across six categories in various foods. The results showed satisfactory separation of the dyes was achieved using a phenyl column, acetonitrile, and a 5 mmol/L ammonium acetate mobile phase. Designing methanol-acetonitrile (2:3, v/v) as a bifunctional extractant can minimize matrix interference and enhance synergistic effects. The LODs of the method were in the range of 0.15-6.28 mu g/L, the LOQs were in the range of 0.48-20.52 mu g/L with good linearity, and the correlation coefficients (r2) were greater than 0.99. The recoveries at the three spiked levels were in the range of 82.6 %-106.3 %. The technique detected four synthetic dyes within regulatory limits in six out of 66 sample batches (9.09 %), demonstrating its effectiveness in addressing interference and strong applicability.

引用

页数：12