Highly sensitive spectrofluorimetric determination of ephedrine hydrochloride in pharmaceutical preparations

被引：0
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作者：
Ulu, Sevgi Tatar [1 ]
机构：
[1] Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul 34452, Turkey
来源：
Journal of AOAC International | 1600年 / 89卷 / 05期
关键词：
A sensitive and specific spectrofluorimetry method was developed and validated for the quantification of ephedrine (EP) in pharmaceutical preparations. The method is based on the fluorescent enhancing reaction of EP with 7-chloro-4-nitrobenzofurazan (NBD-CI; derivatization reagent); in borate buffer of pH 9 to yield a yellow; fluorescent product. Under these experimental conditions; the derivatized product of EP had excitation and emission wavelength maxima at 458 and 516 nm; respectively. The linear range of this method was 20-2500 ng/mL. The detection limit was 7.3 ng/mL EP. Intra- and interday precisions of the assay at 3 concentrations within this range were 0.037-1.77%. The low relative standard deviation values indicate good precision; and high recovery values indicate excellent accuracy of the method. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations; and the results indicate that the method is equally as accurate; precise; and reproducible as the official method;
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Journal article (JA)
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页码：1263 / 1267
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