Alleviation of aluminum toxicity in soybean seedlings by nitric oxide and hydrogen peroxide and analysis of their interaction

被引：0
作者：
Song, Hong-Ming [1 ]
Xing, Cheng-Hua [2 ]
Wu, Kun [1 ]
Wang, Fang-Mei [1 ]
He, Zhen-Quan [3 ]
Cai, Miao-Zhen [3 ]
机构：
[1] College of Chemistry and Life Science, Zhejiang Normal University, Jinhua
[2] Bioengineering Institute, Jinhua College of Vocation and Technology, Jinhua
[3] College of Geography and Environmental Science, Zhejiang Normal University, Jinhua
来源：
| 2014年 / 27卷 / 09期
关键词：
Al toxicity; Hydrogen peroxide; Nitric oxide; Soybean;
D O I：
10.13198/j.issn.1001-6929.2014.09.15
中图分类号：
学科分类号：
摘要：
Nitric oxide (NO) and hydrogen peroxide (H2O2) are key signaling molecules in plants. In recent years, the roles of the two compounds in the tolerance of plants to abiotic stress has received much attention, and increasing evidence indicates that NO and H2O2 play essential roles in aluminum (Al) tolerance. However, the physiological mechanism by which NO and H2O2 alleviate Al toxicity in soybean and their interactions remain unknown. In the present study, we examined the effect of Al on endogenous NO and H2O2 accumulation, the role of exogenous NO and H2O2 in Al tolerance, and enzyme activity related to NO and H2O2 accumulation in soybean root apices. Root elongation and Al content in root tips were used as indicators to detect the effects of NO and H2O2. Al concentration of 50 or 100 μmol/L significantly inhibited root growth, increased Al accumulation in root tips, and promoted endogenous NO production. Treatments with H2O2+Al, sodium nitroprusside SNP+Al, or H2O2+SNP+Al increased relative root length by 64.6%, 63.5% and 69.5%, respectively, and decreased Al content in the root tips by 34.6%, 36.8% and 32.9%, respectively. This showed that an exogenous NO donor SNP and H2O2 both alleviated Al-induced root growth inhibition and Al accumulation in the root apex. Moreover, exogenous NO increased endogenous H2O2 accumulation and decreased peroxidase activity, whereas exogenous H2O2 had no significant effect on endogenous NO production. Collectively, the results showed that NO and H2O2 exhibited a synergistic effect on alleviating Al toxicity in soybean, and NO acted upstream of H2O2 in the signaling pathway through the increment of peroxidase-dependent H2O2 accumulation.
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页码：1061 / 1066
页数：5
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