Glycolysis Capacity of Bovine Muscle during Early Stage of Postmortem Aging under Action of HIF-1α

被引：0
|
作者：
Chen C. [1 ]
Guo Y. [1 ]
Shi X. [1 ]
Guo Z. [1 ]
Ma G. [1 ]
Yu Q. [1 ]
机构：
[1] College of Food Science and Engineering, Gansu Agricultural University, Lanzhou
来源：
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | 2022年 / 53卷 / 04期
关键词：
Bovine muscle; Glycolysis; Hypoxia-inducible factor-1α; Postmortem aging;
D O I：
10.6041/j.issn.1000-1298.2022.04.042
中图分类号：
学科分类号：
摘要：
Aiming to investigate the effect mechanism of hypoxia-inducible factor-1α (HIF-1α) on glycolysis capacity of postmortem bovine muscle, the bovine M. Longissimus dorsi was selected as the experimental material, the cellular aggregation and nuclear migration of HIF-1α, expression of HIF-1α and glycogen transporter-1, glycolysis rate-limiting enzymes activities, glycolytic potential, and pH values were determined at different time points of postmortem aging (0 h, 6 h, 12 h, 24 h, and 48 h) in the control group and 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1)-treated group. The results showed that HIF-1α was aggregated and translocated to the cell nucleus in bovine muscle at the early stage of postmortem aging. The expression levels of HIF-1α were increased by 1.20-fold at 0~6 h (P<0.05), which were significantly higher than those in the YC-1-treated group at 6~48 h (P<0.05). With the steady accumulation of HIF-1α, the expression levels of GLUT-1 were significantly increased by 1.91-fold within 0~6 h in the control group (P<0.05). The hexokinase (HK) and phosphofructokinase (PFK) activities were significantly elevated to 197.43 U/g protein and 27.33 U/g protein at 0~6 h and 0~12 h, respectively (P<0.05). As for the glycolysis capacity, the glycogen consumption and lactate accumulation rates were significantly higher in the control than those in the YC-1-treated group (P<0.05). The glycolytic potential was significantly increased by 1.10-fold at 0~12 h (P<0.05) and was significantly higher than that of YC-1 treatment from 6h to 24 h (P<0.05). Meanwhile, pH values in the control group were significantly decreased to the ultimate pH (pHu) at 0~12 h (P<0.05), which was 12 h earlier than that in the YC-1-treated group. The results indicated that HIF-1α played an important role in enhancing the glycolytic capacity of bovine muscle by inducing GLUT-1 expression and elevating the activities of glycolysis rate-limiting enzymes at the early stage of postmortem aging. © 2022, Chinese Society of Agricultural Machinery. All right reserved.
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页码：403 / 411
页数：8
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