Diversity loss and light limitation threaten the sustainability of ecosystem productivity gains under nitrogen enrichment

Plant photosynthesis significantly regulates atmospheric CO₂ but is often limited by nitrogen (N) availability. While N deposition could alleviate this limitation and enhance gross ecosystem productivity (GEP), its long-term effects are uncertain due to potential negative impacts like biodiversity loss and soil acidification. Yet, many long-term N addition experiments emphasize community biomass over gross GEP. Here, we conducted a six-year N addition experiment in an alpine meadow, frequently monitoring GEP, community structure, aboveground net primary productivity (ANPP)and plant traits. We found that N addition significantly enhanced GEP in the first three years, but during 4–6 years this effect disappeared. We further disentangled the mechanisms affecting GEP into biomass-based and non-biomass-based processes. The latter is expressed as biomass-specific GEP, defined as GEP per unit biomass. Differing with GEP, biomass-specific GEP provides a metric of carbon assimilation efficiency normalized to biomass. Unlike previous studies, we found that it was not ANPP, but specific GEP that determined the loss of the short-term N effect. ANPP showed a consistent increase under N addition, whereas specific GEP decreased in the last three years. This specific GEP reduction was primarily regulated by biodiversity loss and increased light limitation under N addition. Overall, our findings suggest that short-term benefits of N deposition on GEP are not sustained in long term, highlighting the need to explore the non-biomass-based mechanisms to better predict ecosystem responses to prolonged N enrichment. © 2024 Elsevier B.V.