The wavelet transform assisted laser-induced fluorescence detector with high sensitivity

被引：0

作者：

Zheng, Chao ^{[1
,2
]}

Wang, Chuanliang ^{[2
,4
]}

Sha, Tao ^{[2
]}

Wang, Guiqiu ^{[1
]}

Guan, Yafeng ^{[2
,5
]}

He, Hui ^{[3
]}

Jin, Shi ^{[3
]}

Geng, Xuhui ^{[2
]}

机构：

[1] Dalian Maritime Univ, Sch Sci, 1 Linghai Rd, Dalian 116024, Peoples R China

[2] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Key Lab Deep Sea Composit Detect Technol Liaoning, 457 Zhongshan Rd, Dalian 116023, Peoples R China

[3] Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Chinese Acad Sci, Inst Deep Sea Sci & Engn, 28 Luhuitou Rd, Sanya 572000, Peoples R China

来源：

TALANTA | 2025年 / 285卷

关键词：

Laser-induced fluorescence; Inverted suction injection; Wavelet transform; High sensitivity; Good reproducibility; LIGHT-EMITTING DIODE; CAPILLARY-ELECTROPHORESIS; SYSTEM; MICRODEVICE; EXTRACTION; SCALE;

D O I：

10.1016/j.talanta.2024.127257

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A wavelet transform assisted laser-induced fluorescence detector with high sensitivity was developed and evaluated. An unconventional microscope objective with a numerical aperture (NA) of 0.5 and a working distance (WD) of 4.0 mm was employed, which improved fluorescence collection efficiency, increasing SNR to about 5 times. An aspherical lens was used in the collection optical path to reduce the diameter of the fluorescence imaging size, increasing the SNR to about 1.3 times. The "inverted suction injection" method of flow injection analysis (FIA) was used for the first time in LIF evaluation to reduce contamination errors and improve the accuracy of ultra-trace analysis. The wavelet transform method was proposed for data processing of LIF signals, which can reduce noise from 0.009 mV to 0.002 mV without changing the peak height and peak width. The limit of detection (LOD, 3 sigma method) for sodium fluorescein was 9.7 x 10-14 M or 3.9 fluorescein molecules in 68 pL detection volume, which was the lowest level of LIFs evaluated by FIA mode. The LIF combined with capillary electrophoresis (CE) achieved an LOD of 4.9 x 10-15 M for fluorescein sodium, which is the best level that has been reported.

引用

页数：7