Effects of cadmium exposure on gut microbiota and antibiotic resistance genes in Haliotis diversicolor abalone

被引：1
作者：
Yao T. [1 ,2 ]
Ye L. [1 ]
Wang S. [3 ]
Lu J. [1 ]
Li H. [3 ]
Yu G. [1 ]
机构：
[1] Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou
[2] Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Research Center of Hydrobiology, Jinan University, Guangzhou
[3] School of Public Health, Lanzhou University, Lanzhou
来源：
Chemosphere | 2024年 / 352卷
关键词：
Antibiotic resistance genes; Cadmium; Gut microbiome; Haliotis diversicolor; Potential pathogens;
D O I：
10.1016/j.chemosphere.2024.141507
中图分类号：
学科分类号：
摘要：
Heavy metals in soil, water, and industrial production can affect the antibiotic resistance of bacteria. Antibiotic resistance in gut microbiota has been extensively researched. The effects of cadmium (Cd) was investigated on the gut microbiota and antibiotic resistance genes (ARGs) of Haliotis diversicolor, a commercially important abalone species. By exposing H. diversicolor to four concentrations of Cd (0 μg L−1 (control), 6.5 μg L−1 (low), 42.25 μg L−1 (medium), and 274.63 μg L−1 (high)) for 30 and 60 days, 16 types of ARG (aadA-01, aadA-02, cfr, dfrA1, ermB, floR, folA, mecA, sul2, tetB-01, tetC-01, tetD-01, tetG-01, tetM-02, tetQ, vanC-01), and 1213 genus and 27 phylum microbiomes were detected. ARGs can be resistant to aminoglycoside, beta-lactamase, macrolide-lincosamide-streptogramin B, multidrug, florfenicol, macrolide, sulfonamides, tetracyclines, and vancomycin. Cadmium exposure significantly alters the abundance of tetC-01, tetB-01, tetQ, sul2, and aadA-01. About 5% (61) of genus-level microorganisms were significantly affected by Cd exposure. Microbiota alpha and beta diversities in the 60-day 42.25 μg L−1 Cd treatment differed significantly from those in other treatments. In addition, 26 pathogens were detected, and two pathogens (Vibrio and Legionella) were significantly affected by Cd exposure. Significant correlations between pathogens and ARGs increased with increased Cd concentration after 60 days of Cd exposure. Cadmium exposure may cause gut microbiota disturbance in H. diversicolor and increase the likelihood of ARG transfer to pathogens, increasing potential ecological and economic risks. © 2024 Elsevier Ltd
引用
收藏
相关论文
共 94 条
  • [1] Anderson M.J., Permutational Multivariate Analysis of Variance (PERMANOVA), (2017)
  • [2] Atlanta G., Toxicological Profile for Cadmium, 2012, (2012)
  • [3] Auffret M.D., Dewhurst R.J., Duthie C.A., Rooke J.A., Wallace R.J., Freeman T.C., Stewart R., Watson M., Roehe R., The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle, Microbiome, 5, pp. 1-11, (2017)
  • [4] Awofolu O.R., A survey of trace metals in vegetation, soil and lower animal along some selected major roads in metropolitan city of Lagos, Environ. Monit. Assess., 105, pp. 431-447, (2005)
  • [5] Baker-Austin C., Wright M.S., Stepanauskas R., McArthur J.V., Co-selection of antibiotic and metal resistance, Trends Microbiol., 14, pp. 176-182, (2006)
  • [6] Bastian M., Heymann S., Jacomy M., Gephi: an open source software for exploring and manipulating networks, Proceedings of the international AAAI conference on web and social media, 3, 1, pp. 361-362, (2009)
  • [7] Bednorz C., Oelgeschlager K., Kinnemann B., Hartmann S., Neumann K., Pieper R., Bethe A., Semmler T., Tedin K., Schierack P., Wieler L.H., Guenther S., The broader context of antibiotic resistance: zinc feed supplementation of piglets increases the proportion of multi-resistant Escherichia coli in vivo, Int. J. Med. Microbiol., 303, pp. 396-403, (2013)
  • [8] Binh C.T.T., Heuer H., Kaupenjohann M., Smalla K., Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids, FEMS Microbiol. Ecol., 66, pp. 25-37, (2008)
  • [9] Breton J., Daniel C., Dewulf J., Pothion S., Froux N., Sauty M., Thomas P., Pot B., Foligne B., Gut microbiota limits heavy metals burden caused by chronic oral exposure, Toxicol. Lett., 222, pp. 132-138, (2013)
  • [10] Caporaso J.G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F.D., Costello E.K., Et al., QIIME allows analysis of high-throughput community sequencing data, Nat. Methods, 7, pp. 335-336, (2010)
12345678910