Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L.

被引：0

作者：

机构：

[1] Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, Shandong

[2] Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, Shandong

[3] Tobacco Research Institute of Hubei Province, Wuhan

[4] Zunyi Municipal Tobacco Company, Zunyi, 563000, Guizhou

[5] Baoshan Oriental Tobacco Company, Baoshan, 678000, Yunnan

来源：

Wu, Xia | 1600年 / State Tobacco Monopoly Bureau and China Tobacco Society卷 / 20期

关键词：

Abiotic stress; Biotic stress; Catalase; Expression analysis; Nicotiana tabacum;

D O I：

10.3969/j.issn.1004-5708.2014.05.017

中图分类号：

学科分类号：

摘要：

The full-length cDNA of Catalase 1 (CAT1) from Nicotiana tabacum var. NC89 was cloned based on specific primers designed according to CAT1 mRNA sequences in other plants. An amino acid sequence alignment indicated that CAT1 contains 492 amino acid residues. Phylogenetic analysis showed that NC89 CAT1 shares high similarity with gene from N. benthamiana. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that CAT1 is highly expressed in leaf and calyx, while less expressed in roots and seeds. The expression of NC89 CAT1 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought, and TMV infection, whereas it was inhibited by ultraviolet irradiation. It was suggested that CAT1 plays an important role in growth, development, and interplay of abiotic and biotic stresses in plant. ©, 2014, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

引用

页码：103 / 109

页数：6

共 28 条

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