Agrobacterium-mediated genetic transformation in cucumber (Cucumis sativus L.)

被引：0
作者：
Vasudevan, A. [1 ]
Selvaraj, N. [2 ]
Ganapathi, A. [3 ]
Choi, C.W. [1 ]
机构：
[1] Department of Biology and Medicinal Science, Pai Chai University
[2] Department of Botany, Periyar E. V.R. College (Autonomous), Tiruchirappalli 620023, T. N.
[3] Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620024, T. N.
来源：
American Journal of Biochemistry and Biotechnology | 2007年 / 3卷 / 01期
关键词：
Agrobacterium; Cotyledon; Cucumber; GUS expression;
D O I：
10.3844/ajbbsp.2007.24.32
中图分类号：
学科分类号：
摘要：
The present study was undertaken to develop efficient transformation protocol for cucumber cv. Poinsett 76 using Agrobacterium strain EHA 105. Five-day-old mature cotyledon explants was used for transformation study. The infected explants were co-cultivated for 2 days in MS medium containing BA (1.0 mg L-1). The selection of transformed shoots was carried out in MS medium fortified with BA (1.0 mg L-1), Cefotaxime (300 mg L -1) and PPT (2.0 mg L-1). The transformed shoots were elongated in MS medium containing BA (1.0 mg L-1), Cefotaxime (300 mg L-1), PPT (2.0 mg L-1) along with GA3 (0.5 mg L-1). The rooting of elongated shoots was achieved in MS medium with BA (1.0 mg L-1), Cefotaxime (300 mg L-1), PPT (2.0 mg L-1) and IBA (0.6mg L-1). The transient GUS expression assay and leaf disc assay were carried out in order to find transformed shoots. The molecular confirmation of transformed shoots revealed the foreign gene integration into cucumber genome. © 2007 Science Publications.
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页码：24 / 32
页数：8
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共 42 条
  • [1] Kung S.D., Introduction: From Hybrid Plants to Transgenic Plants, Transgenic Plants, Engineering and Utilization, 1, pp. 1-12, (1993)
  • [2] Suzuki S., Murai N., James N., Burnell R., Arai R., Changes in photosynthetic carbon flow in transgenic rice plants that express C4-Type Phosphoenolpyruvate Carboxykinase from Urochloa panicoides, Plant Physiol, 124, pp. 163-172, (2000)
  • [3] Daniell H., Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants, Trends in Plant Sci, 6, pp. 219-226, (2001)
  • [4] Aharon R., Shahak Y., Wininger S., Bendov R., Kapulnik Y., Galili G., Over expression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress, Plant Cell, 15, pp. 439-447, (2003)
  • [5] Gamborg O.L., Davis P., Stahelut R.W., Somatic embryogenesis in cell suspension cultures of glycine species, Plant Cell Rep, 2, pp. 209-212, (1968)
  • [6] Oridate T., Atsumi H., Ito S., Araki H., Genetic difference in somatic embryogenesis from seed in melon (Cucumis melo L.), Plant Cell Tiss. Org. Cult, 29, pp. 27-30, (1992)
  • [7] Walden R., Wingender R., Gene-Transfer and Plant- An easy-going general review. Regeneration Techniques, Trends. Biotech, 13, pp. 324-331, (1995)
  • [8] Den Nijs A.P.M., Custers J.B.M., Introduction resistance into cucumbers by interspecific hybridization, Crop Improvement and Protection, pp. 382-396, (1990)
  • [9] Chee P.P., Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants, Plant Cell Rep, 9, pp. 245-248, (1990)
  • [10] Chee P.P., Slightom J.L., Transfer and expression of Cucumber Mosaic Virus Coat protein gene in the genome of Cucumis sativus, J. Am. Soc. Hortic. Sci, 116, pp. 1098-1102, (1991)
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