Process optimization of exopolysaccharides from the fermentation broth of paecilomyces hepiali by ultrafiltration

被引：0

作者：

Wu, Zhongwei ^{[1
,2
]}

Zhang, Mingxia ^{[1
]}

Lu, Junwen ^{[2
]}

Yang, Zijiang ^{[2
]}

Sun, Jin ^{[2
]}

Zhao, Kun ^{[2
]}

Zeng, Xiaoxiong ^{[2
]}

机构：

[1] College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, Henan

[2] College of Food Science and Technology, Nanjing Agricultural University, Nanjing

来源：

Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 12期

关键词：

Exopolysaccharide; Optimization; Paecilomyces hepiali; Ultrafiltration;

D O I：

10.16429/j.1009-7848.2015.12.016

中图分类号：

学科分类号：

摘要：

In order to acquire the technological parameters of ultrafiltration membrane separation exopolysaccharide from the fermentation broth of Paecilomyces hepiali. In this study, the trapping effects of membranes of different molecular weight cut-off with the tangential flow ultrafiltration system were compared. The results showed that the exopolysaccharide in the fermentation broth can be divided into three segments (>2 000ku, 100-2 000 ku and <100ku) when using the membrane of 0.1 μm and 100 ku in order. Furthermore, the effect of material to water, operation pressure and pH on membrane flux were tested using 100 ku membrane module by one-factor-at-a-time and orthogonal design. The optimal operation conditions were material to water 3:2, 0.2 MPa pressure and pH 8.5, and the membrane flux and trapped polysaccharide were 19.5 L·m2·h-1 and 122.47 g·m2·h-1. In the trapped fluid the purity of 800 ku polysaccharide fraction was 89.7%. Compared with the traditional separation method, ultrafiltration by membrane possessing a series advantage of lower energy expenditure, loss percentage and higer performance. © 2015, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：115 / 121

页数：6

共 14 条

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