Process optimization of exopolysaccharides from the fermentation broth of paecilomyces hepiali by ultrafiltration

被引：0

作者：

Wu, Zhongwei ^{[1
,2
]}

Zhang, Mingxia ^{[1
]}

Lu, Junwen ^{[2
]}

Yang, Zijiang ^{[2
]}

Sun, Jin ^{[2
]}

Zhao, Kun ^{[2
]}

Zeng, Xiaoxiong ^{[2
]}

机构：

[1] College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, Henan

[2] College of Food Science and Technology, Nanjing Agricultural University, Nanjing

来源：

Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 12期

关键词：

Exopolysaccharide; Optimization; Paecilomyces hepiali; Ultrafiltration;

D O I：

10.16429/j.1009-7848.2015.12.016

中图分类号：

学科分类号：

摘要：

In order to acquire the technological parameters of ultrafiltration membrane separation exopolysaccharide from the fermentation broth of Paecilomyces hepiali. In this study, the trapping effects of membranes of different molecular weight cut-off with the tangential flow ultrafiltration system were compared. The results showed that the exopolysaccharide in the fermentation broth can be divided into three segments (>2 000ku, 100-2 000 ku and <100ku) when using the membrane of 0.1 μm and 100 ku in order. Furthermore, the effect of material to water, operation pressure and pH on membrane flux were tested using 100 ku membrane module by one-factor-at-a-time and orthogonal design. The optimal operation conditions were material to water 3:2, 0.2 MPa pressure and pH 8.5, and the membrane flux and trapped polysaccharide were 19.5 L·m2·h-1 and 122.47 g·m2·h-1. In the trapped fluid the purity of 800 ku polysaccharide fraction was 89.7%. Compared with the traditional separation method, ultrafiltration by membrane possessing a series advantage of lower energy expenditure, loss percentage and higer performance. © 2015, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：115 / 121

页数：6

共 14 条

[1] Donot F., Fontana A., Baccou J.C., Et al., Microbial exopolysaccharides: Main examples of synthesis, excretion, genetics and extraction, Carbohyd Polym, 87, 2, pp. 951-962, (2012)
[2] Yan J.K., Li L., Wang Z.M., Et al., Acidic degradation and enhanced antioxidant activities of exopolysaccharides from Cordyceps sinensis mycelial culture, Food Chem, 117, 4, pp. 641-646, (2009)
[3] Huang T.T., Lin J., Cao J.F., Et al., An exopolysaccharide from Trichoderma pseudokoningii and its apoptotic activity on human leukemia K562 cells, Carbohyd Polym, 89, 2, pp. 701-708, (2012)
[4] Cho E.J., Hwang H.J., Kim S.W., Et al., Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumiiin ob/ob mice, Appl Microbiol Biotechnol, 75, 6, pp. 1257-1265, (2007)
[5] Song D., He Z.Y., Wang C.H., Et al., Regulation of the exopolysaccharide from an anamorph of Cordyceps sinensis on dendritic cell sarcoma (DCS) cell line, Eur J Nutr, 52, 2, pp. 687-694, (2013)
[6] Zhu J.S., Gao L., Li X.H., Et al., Maturational alteration of oppositely orientated rDNA and differential proliferation of GC- and AT-biased genotypes of Ophiocordyceps sinensis and Paecilomyces hepiali in natural Cordyceps sinensis, Am J Biomed Sci, 2, 3, pp. 217-238, (2010)
[7] Zhou X.W., Gong Z.H., Su Y., Et al., Cordyceps fungi: natural products, pharmacological functions and developmental products, J Pharm Pharmacol, 61, 3, pp. 279-291, (2009)
[8] Huang Q.L., Siu K.C., Wang W.Q., Et al., Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus, Process Biochem, 48, 2, pp. 380-386, (2013)
[9] Wu Z.W., Lu J.W., Wang X.Q., Et al., Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali, Carbohyd Polym, 99, 1, pp. 226-234, (2014)
[10] Sheng L., Chen J.P., Li J., Et al., An exopolysaccharide from cultivated Cordyceps sinensis and its effects on cytokine expressions of immunocytes, Appl Biochem Biotechnol, 163, 5, pp. 669-678, (2011)

← 1 2 →