In situ remediation of low-permeability soils contaminated with trichloroethylene (TCE) is challenging due to limited mass transfer and low bioavailability in clay soils. The electrokinetic-enhanced bioremediation (EK-BIO) system offers a promising solution by combining electrokinetics with bioremediation to address these challenges. While previous studies have demonstrated microbial succession and TCE removal, the long-term performance of dechlorination and interactions between electrode reactions and anaerobic dechlorination remain unclear. This study constructed five one-dimensional columns, each operated for a different period (28, 42, 56, 84 and 138 days) to explore spatial and temporal dechlorination patterns. Continuous TCE degradation was achieved, with 46.52 % of TCE recovery. Prolonged electrokinetic operation accelerated the first-step dehalogenation (TCE to DCE). Although Dehalococcoides was widespread at 138 days (2.30-5.74 %), oxygen exposure led to irreversible damage, necessitating secondary inoculation. The presence of aerobic bacteria (Comamonas and Pseudomonas) suggested the formation of aerobic detoxification pathways in electrode chambers. Gene expression analysis (tceA, vcrA and Dhc16S) further confirmed the loss of 2nd and 3rd step dehalogenation (DCE to ethene) over time. These findings demonstrate that secondary inoculation and alternative aerobic pathways can sustain long term biodegradation in the EK-BIO system. This study highlights the potential of the EK-BIO system for effective remediation of TCE-contaminated low-permeability soils, supporting its field application.
