Gene transfection of bioactive glass fibers

被引：0
作者：
机构：
[1] School of Materials Science and Engineering, South China University of Technology, Guangzhou
[2] National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou
[3] Key Laboratory of Biomedical Engineering in Guangdong, Guangzhou
来源：
Chen, Xiao-Feng | 1600年 / Science Press卷 / 29期
基金：
中国国家自然科学基金;
关键词：
Bioactive glass; Calcium phosphate; Fiber; Gene transfection;
D O I：
10.15541/jim20140074
中图分类号：
学科分类号：
摘要：
Bioactive glasses (BGs) exhibit potential applications for gene transfection because of their composition including Ca and P. Here, bioactive glass fibers (BGFs) with mesopores or hierarchical nanopores, were prepared by electrospinning process using BGs Sol-Gel precursor and its effect on mediating gene transfection was investigated. The results indicate that BGF acts as a gene vector by releasing Ca2+ and PO43-, and then reunioning them along with plasmid DNA in Dulbecco's Modified Eagle's Medium (DMEM). BGFs have a dose-dependent manner in transfection efficiency. When using 1 μg/mL plasmid, the transfection efficiency of BGF with concentration at 1000 μg/mL is higher than 50% of lipofectamine LTX_PLUS. The transfection mechanism of BGF is similar to that of calcium phosphate (CaP) system. Furthermore, BGF's sufficient ions releasing ensures stability and effectiveness to be applied in gene transfection, which makes BGF a promising candidate for gene delivery in replace of traditional gene carrier, the nano-calcium phosphate system.
引用
收藏
页码：1023 / 1028
页数：5
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