Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy

被引：0
作者：
Zhang, Shi-Chao [1 ,2 ,3 ]
Li, Si-Min [1 ]
Yang, Guang [1 ]
Li, Hui [1 ]
Xiong, Da-Xi [1 ]
机构：
[1] Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, Jiang Su
[2] Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun
[3] University of Chinese Academy of Sciences, Beijing
来源：
Guangzi Xuebao/Acta Photonica Sinica | 2015年 / 44卷 / 10期
关键词：
Calibration curves; Cylindrical lenses; Fluorescence-microscopy; Super resolution; Three-dimensiona;
D O I：
10.3788/gzxb20154410.1017003
中图分类号：
学科分类号：
摘要：
The relationship between the ellipticity of point spread function and the focal length of cylindrical lens was investigated. Fluorescent inverted microscopey imaging system is based on Olympus IX-83. With three different focal length, the point spread function with custom built stochastic optical reconstruction microscopy instruments was measured. A method to evaluate the cylindrical lens was developed based on linear region and localization error. The results show that linear region of 1 μm and axial localization error of 16 nm can be achieved with correct focal length. As the demonstration, three dimension super-resolution image of Actin filaments was reconstructed. ©, 2015, Chinese Optical Society. All right reserved.
引用
收藏
页数：6
相关论文
共 16 条
  • [11] Grover G., Deluca K., Quirin S., Et al., Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE), Optics Express, 20, 24, pp. 26681-26695, (2012)
  • [12] Huang B., An in-depth view, Nature Methods, 8, 4, pp. 304-305, (2011)
  • [13] Small A., Stahlheber S., Fluorophore localization algorithms for super-resolution microscopy, Nature Methods, 11, 3, pp. 267-279, (2014)
  • [14] Allen J.R., Ross S.T., Davidson M.W., Single molecule localization microscopy for superresolution, Journal of Optics, 15, 9, (2013)
  • [15] Henriques R., Lelek M., Fornasiero E.F., Et al., QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ, Nature Methods, 7, 5, pp. 339-340, (2010)
  • [16] Metcalf D.J., Edwards R., Kumarswami N., Et al., Test samples for optimizing STORM super-resolution microscopy, Jove-Journal of Visualized Experiments, 79, (2013)
12