Recently;
we reported the anti-inflammatory effects of arvelexin isolated from Brassica rapa in macrophages. In the present study;
the effects of arvelexin were investigated in a dextran sulfate sodium (DSS)-induced colitis mouse model and in a cellular model. In the DSS-induced colitis model;
arvelexin significantly reduced the severity of colitis;
as assessed by disease activity;
colonic damage;
neutrophil infiltration;
and levels of colonic iNOS. Moreover;
arvelexin inhibited the expressions of IL-8;
IP-10;
ICAM-1;
and VCAM-1 in HT-29 colonic epithelial cells. Arvelexin also inhibited the TNF-α-induced adhesion of U937 monocytic cells to HT-29 cells. Furthermore;
arvelexin reduced p65 NF-κB subunit translocation to the nucleus and IκBα degradation in the colonic tissues and in TNF-α-induced HT-29 cells. These results demonstrate that the ameliorative effects of arvelexin on colonic injury are mainly related to its ability to inhibit the inflammatory responses via NF-κB inactivation;
and support its possible therapeutic role in colitis. © 2012 American Chemical Society;