Application of Time-resolved Fluorescence Immunoassay to Concurrently Quantify Fumonisin B1, B2 and B3 in Corn

被引：0
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作者：
Zhang J. [1 ,2 ]
Ma L. [1 ,2 ]
Hou L. [1 ]
Menghenaren [3 ]
Wen K. [4 ]
Hou X. [1 ]
机构：
[1] College of Animal Science and Technology, Beijing University of Agricultural, Beijing
[2] Beijing WDWK Biotechnology Co., Ltd., Beijing
[3] Chifeng City Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement Detachment, Chifeng
[4] College of Veterinary Medicine, China Agricultural University, Beijing
来源：
Science and Technology of Food Industry | 2024年 / 45卷 / 13期
关键词：
corn; fumonisin B[!sub]1[!/sub; fumonisin B[!sub]2[!/sub; fumonisin B[!sub]3[!/sub; quantitative detection; time-resolved fluorescent microsphere;
D O I：
10.13386/j.issn1002-0306.2023080334
中图分类号：
学科分类号：
摘要：
This study aimed to establish a time-resolved fluorescence immunoassay method for the simultaneous quantitative detection of fumonisin B1, B2 and B3 in corn. The EDC/NHS active ester method was used to prepare time-resolved fluorescent microspheres and fumonisin antibody conjugates. Fumonisin antigen was immobilized on a nitrocellulose membrane as the test line. By optimizing the number of test line, a competitive immunoassay method for detecting fumonisin was eventually established. Subsequently, the sensitivity, specificity, accuracy, precision, and correlation with the national standard method were evaluated for the established method. The detection limits of the established method by using two test line pattern were 107.68~168.28 μg/kg, and the quantification limits were 283.46~444.63 μg/kg. The cross-reactivity with fumonisin B1, fumonisin B2 and fumonisin B3 were 100%, 85.59% and 72.72%, respectively, and no significant cross-reactivity was observed with five other common mycotoxins. The recovery rates ranged from 88.37% to 117.42%, and the coefficients of variation were lower than 10%. The conformity of the results obtained by the established method, as compared to the immunoaffinity column purification-high performance liquid chromatography (IAC-HPLC) method specified in the national standard GB 5009.240-2016, fell within the range of 92.17% to 107.21%. The established time-resolved fluorescence immunoassay method meets the requirements for rapid on-site quantitative detection of fumonisin B1, B2 and B3 in corn. © The Author(s) 2024. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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页码：265 / 271
页数：6
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