Studying microbially induced corrosion on glass using ToF-SIMS

被引:3
作者
Parker, Gabriel D. [1 ]
Plymale, Andrew [3 ]
Hager, Jacqueline [3 ]
Hanley, Luke [1 ]
Yu, Xiao-Ying [2 ]
机构
[1] Univ Illinois, Dept Chem, 845 W Taylor St, Chicago, IL 60607 USA
[2] Oak Ridge Natl Lab, Mat Sci & Technol Div, Phys Sci Directorate, 1 Bethel Valley Rd, Oak Ridge, TN 37830 USA
[3] Pacific Northwest Natl Lab, Energy & Environm Directorate, 902 Battelle Blvd, Richland, WA 99354 USA
关键词
MICROBIOLOGICALLY INFLUENCED CORROSION; ION MASS-SPECTROMETRY; CONTINUOUS WAVELET TRANSFORM; PSEUDOMONAS-AERUGINOSA; PAENIBACILLUS-POLYMYXA; MULTIVARIATE-ANALYSIS; BIOFILM FORMATION; PEAK DETECTION; FATTY-ACIDS; FLUID-FLOW;
D O I
10.1116/6.0003883
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Microbially induced corrosion (MIC) is an emerging topic that has huge environmental impacts, such as long-term evaluation of microbial interactions with radioactive waste glass, environmental cleanup and disposal of radioactive material, and weathering effects of microbes. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), a powerful mass spectral imaging technique with high surface sensitivity, mass resolution, and mass accuracy, can be used to study biofilm effects on different substrates. Understanding how to prepare biofilms on MIC susceptible substrates is critical for proper analysis via ToF-SIMS. We present here a step-by-step protocol for preparing bacterial biofilms for ToF-SIMS analysis, comparing three biofilm preparation techniques: no desalination, centrifugal spinning (CS), and water submersion (WS). Comparisons of two desalinating methods, CS and WS, show a decrease in the media peaks up to 99% using CS and 55% using WS, respectively. Proper desalination methods also can increase biological signals by over four times for fatty acids using WS, for example. ToF-SIMS spectral results show chemical compositional changes of the glass exposed in a Paenibacillus polymyxa SCE2 biofilm, indicating its capability to probe microbiologically induced corrosion of solid surfaces. This represents the proper desalination technique to use without significantly altering biofilm structure and substrate for ToF-SIMS analysis. ToF-SIMS spectral results showed chemical compositional changes of the glass exposed by a Paenibacillus bacterial biofilm over 3-month inoculation. Possible MIC products include various phosphate phase molecules not observed in any control samples with the highest percent increases when experimental samples were compared with biofilm control samples.
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页数:15
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