Probe-Type High-Sensitivity DNA Hybridization Detection Based on Optical Fiber SPR

被引：0
作者：
Li, Xuegang [1 ]
Gong, Pengqi [1 ]
Zhou, Xue [1 ]
机构：
[1] Northeastern Univ, Coll Informat Sci & Engn, Shenyang 110819, Peoples R China
来源：
IEEE SENSORS JOURNAL | 2024年 / 24卷 / 24期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Sensors; DNA; Optical surface waves; Optical fiber sensors; Probes; Surface morphology; Gold; Surface waves; Surface treatment; Optical films; Deoxyribonucleic acid (DNA) hybridization; optical fiber; surface plasmon resonance (SPR); BIOSENSOR; SENSOR;
D O I：
10.1109/JSEN.2024.3484011
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
Deoxyribonucleic acid (DNA) detection represents an important direction of diagnosis and treatment of genetic diseases, which contains all the genetic information of the human body. The DNA sequence remains extremely challenging to detect, despite numerous previous studies that have achieved selective detection, due to the backward sensing scheme. For low-cost, high-sensitivity DNA hybridization detection, a probe-type highly sensitive DNA detection method based on surface plasmon resonance (SPR) is demonstrated in this article. First, low-cost refractive index (RI) tracking detection can be completed via optical fiber SPR. Then, the selective detection of complementary DNA (cDNA) is achieved by binding the probe DNA (pDNA) sequence to the optical fiber SPR sensor using polyethyleneimine (PEI). Compared with previous studies, the sensitivity [0.13 nm/(nmol/L)] is greatly upgraded, and the detection limit (DL) is reach to 4.87 nmol/L. This low-cost and high-performance optical fiber sensing method is conducive to the further development of DNA hybridization detection technology.
引用
收藏
页码：40904 / 40911
页数：8
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