Biomimetic bilayer scaffold from Bombyx mori silk materials for small diameter vascular applications in tissue engineering

被引:0
作者
Castrillon, Ana M. Gaviria [1 ]
Wray, Sandra [2 ]
Rodriguez, Anibal [2 ]
Fajardo, Sahara Diaz [1 ]
Machain, Victoria A. [3 ]
Parisi, Julieta [4 ]
Bosio, Gabriela N. [5 ]
Kaplan, David L. [6 ]
Restrepo-Osorio, Adriana [1 ]
Bosio, Valeria E. [3 ,6 ]
机构
[1] Univ Pontificia Bolivariana, Grp Invest Nuevos Mat GINUMA, Medellin, Colombia
[2] Inst Tecnol Buenos Aires ITBA, Dept Ciencias Vida, Buenos Aires, Argentina
[3] Univ Nacl La Plata, Biometrials Tissue Engieeering Lab, BIOMIT Lab, Inst Fis La Plata,CONICET, La Plata, Buenos Aires, Argentina
[4] Inst Multidisciplinario Biol Celular CICPBA CONICE, Sect Cult Celulares, La Plata, Argentina
[5] Univ Nacl la Plata, CONICET, Inst Invest Fisi Quim Teor & Aplicadas INIFTA, La Plata, Buenos Aires, Argentina
[6] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
关键词
biomimetic; silk fibroin; small diameter; tissue engineering; vascular scaffolds; MATRIX; FIBERS;
D O I
10.1002/jbm.a.37789
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Enhancing the biocompatibility and mechanical stability of small diameter vascular scaffolds remain significant challenges. To address this challenge, small-diameter tubular structures were electrospun from silk fibroin (SF) from silk textile industry discarded materials to generate bilayer scaffolds that mimic native blood vessels, but derived from a sustainable natural material resource. The inner layer was obtained by directly dissolving SF in formic acid, while the middle layer (SF-M) was achieved through aqueous concentration of the protein. Structural and biological properties of each layer as well as the bilayer were evaluated. The inner layer exhibited nano-scale fiber diameters and 57.9% crystallinity, and degradation rates comparable with the SF-M layer. The middle layer displayed micrometer-scale fibers diameters with an ultimate extension of about 274%. Both layers presented contact angles suitable for cell growth and cytocompatibility, while the bilayer material displayed an intermediate mechanical response and a reduced enzymatic degradation rate when compared to each individual layer. The bilayer material emulates many of the characteristics of native small-diameter vessels, thereby suggesting further studies towards in vivo opportunities.
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页数:12
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