A comprehensive review on human enteric viruses in water: Detection methods, occurrence, and microbial risk assessment

Human enteric viruses, such as norovirus, adenovirus, rotavirus, and enterovirus, are crucial targets in controlling biological contamination in water systems worldwide. Due to their small size and low concentrations in water, effective virus concentration and detection methods are essential for ensuring microbial safety. This paper reviews the typical and innovative methods for concentrating and detecting human enteric viruses, highlights viral contamination levels across different water bodies, and discusses the removal efficiencies of virus through various treatment technologies. The application and current gaps of quantitative microbial risk assessment (QMRA) for evaluating the risks of human enteric viruses is also explored. Innovative methods such as digital polymerase chain reaction and isothermal amplification show promise in sensitivity and convenience, however, distinguishing between infectious and non-infectious viruses should be a key focus of future detection techniques. The highest concentrations of human enteric viruses were detected in wastewater, ranging from 103 to 106 copies/L, while drinking water showed significantly lower concentrations, often below 102 copies/L. QMRA studies suggest that exposure to human enteric viruses, whether through contaminated drinking water,occupational contact, or accidental wastewater discharge, could result in a life expectancy of 1.96 x 10-4 to 4.53 x 10-1 days/year.