Lipid-Domain-Selective Assembly of Photosynthetic Membrane Proteins into Solid-Supported Membranes

被引：0
|
作者：
Sumino A. [1 ]
Takeuchi T. [1 ]
Kondo M. [1 ]
Dewa T. [1 ]
Hashimoto H. [2 ,3 ]
Gardiner A.T. [4 ]
Cogdell R.J. [4 ]
Nango M. [1 ]
机构：
[1] Graduate School of Engineering, Nagoya Institute of Technology, Nagoya
[2] Department of Physics, Graduate School of Science, Osaka City University, Osaka, 558-8585, 3-3-138, Sugimoto, Sumiyoshi-ku
[3] CREST/JST, Tokyo
[4] University of Glasgow, Glasgow
来源：
Green Energy and Technology | 2010年 / 44卷
关键词：
AFM; Light-harvesting complex; Lipid domain; Photosynthetic membrane protein; Protein assembly; Supported lipid bilayer; TIRF microscopy;
D O I：
10.1007/978-4-431-99779-5_18
中图分类号：
学科分类号：
摘要：
In a bacterial photosynthesis, light-harvesting complex 2 (LH2) and the light-harvesting-reaction center complex (LH1-RC) play the key roles of capturing and transferring light energy and subsequent charge separation. Here, we present a novel strategy to assemble both LH2 and LH1-RC into a solid-supported lipid bilayer. Spectroscopic and microscopic analyses revealed that both LH2 and LH1-RC were incorporated into the solid-supported lipid bilayer without denaturation. Through a stepwise-assembling procedure, LH2 and LH1-RC were separately organized in the lipid-domain-structured area. © Springer 2010.
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收藏
页码：123 / 128
页数：5
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