Extraction, physicochemical properties and bioactivities of polysaccharides from Conioselinum vaginatium

被引：0

作者：

Li, Wenwen ^{[1
]}

Lin, Yonggang ^{[1
]}

Bian, Peng ^{[1
]}

Pang, Juanxia ^{[1
]}

Wang, Junlong ^{[1
]}

Shi, Wenpan ^{[1
]}

Nuer, Maimaiti ^{[1
]}

机构：

[1] Key Laboratory of Microbial Resources Conservation and Development and Utilization, Yili Normal University, Xinjiang, Yining

来源：

Jingxi Huagong/Fine Chemicals | 2024年 / 41卷 / 09期

关键词：

antioxidant activities; Conioselinum vaginatium polysaccharides; extraction process; hypoglycemic activities; modernization technology of traditional Chinese medicine; physicochemical properties;

D O I：

10.13550/j.jxhg.20230800

中图分类号：

学科分类号：

摘要：

Polysaccharides were extracted from Conioselinum vaginatium via ultrasonic-assisted ionic liquid [1-ethyl-3-methylimidazole bromide (EMIMBr)], with the optimal extraction process optimized. The crude polysaccharides of Conioselinum vaginatium (CV-1), with impurities such as protein, pigment and small molecules removed, were characterized by FTIR, SEM and UV-Vis absorption spectroscopy, and were further evaluated for its in vitro antioxidant and hypoglycemic capacities. The results showed that under the optimal extraction process of material-liquid ratio (the ratio of Conioselinum vaginatium dry powder mass to distilled water volume, g∶mL) 1∶50, pectinase content (based on the mass of Conioselinum vaginatium dry powder, the same below) 0.6%, sonication time 115 min, sonication temperature 55 ℃, enzyme digestion time 90 min, ionic liquid mass concentration 4 g/L, polysaccharides extracted from Conioselinum vaginatium reached (125.278±0.707) mg/g. CV-1 showed a weight-average relative molecular mass of 17570, and mainly consisting of 5 kinds of monosaccharide (molar fraction): fucose (40.83%), rhamnoose (28.03%), arabinose (18.40%), glucose (6.80%) and mannose (5.94%), but very little protein and uronic acid. Meanwhile, CV-1 was an amorphous and crystalline α-configurational glycoside with trihelical conformation, and displayed a lamellar structure with a flat local surface and a mass loss rate of 81.55% at 550 ℃. The median inhibition concentrations (IC50) for diammonium 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate) radical and hydroxyl radical were 1.122 and 1.373 g/L, respectively, and those for α-glucosidase and α-amylase were 14.799 and 15.739 g/L. © 2024 Fine Chemicals. All rights reserved.

引用

页码：1966 / 1977and2081

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