Biodiversity of aquatic invertebrates based on environmental DNA metabarcoding technology: A case study of Lake Haizhu in Guangzhou

被引：0
|
作者：
Tang S. [1 ]
Wang Q. [1 ]
Liu L. [1 ]
Yang Y. [1 ]
机构：
[1] Department of Ecology, Key Laboratory of Philosophy and Social Science in Guangdong Province of Jinan University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou
来源：
Hupo Kexue/Journal of Lake Sciences | 2023年 / 35卷 / 04期
关键词：
biodiversity; environmental DNA; Guangzhou Haizhu National Wetland Park; invertebrates; zooplankton;
D O I：
10.18307/2023.0437
中图分类号：
学科分类号：
摘要：
The diversity and community structure of invertebrates in the water and sediment of Lake Haizhu in the Guangzhou Haizhu National Wetland Park was investigated based on environmental DNA metabarcoding technology (eDNA), and the detection ability for zooplankton was compared between eDNA and traditional morphological identification. The results showed that a total of 9 phyla, 16 classes, 34 orders, 71 families, 93 genera and 137 operational laxonomic units fOTUs) of aquatic invertebrates were detected by eDNA. In the samples directly collected from water (W), a total of 9 phyla, 50 genera and 68 OTUs were detected; in the samples collected by a planktonic net (N), a total of 6 phyla, 27 genera and 35 OTUs were detected; and in the sedi-mental samples (S), a total of 9 phyla, 70 genera and 103 OTUs were detected. Rotifera and Arthropoda were the major invertebrates in both W and N; Annelida and Arthropoda were the major invertebrates in S. The OTU number was highest in S, followed by W and N. The copepods (100%) and most of rotifers (58.82%) identified by the morphological method could be annotated by eDNA, while most of the copepods (71.43%) and rotifers (58.82%>) annotated by eDNA were not detected by the morphological identification; and cladocerans was not annotated by eDNA. The results suggest that eDNA has high potential for invertebrate surveys, and the combination of eDNA and traditional morphological identification provides a more comprehensive understanding of invertebrates and aquatic biodiversity of the lake ecosystem. © 2023 Science Press. All rights reserved.
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页码：1443 / 1456
页数：13
相关论文
共 61 条
  • [1] Wang M, Yuan Y, Yu HY, Et al., Construction of barcode library of freshwater macroinvertebrate in China, Environmental Monitoring in Chi-na, 38, 1, pp. 36-44, (2022)
  • [2] Vehkaoja M, Niemi M, Vaananen VM., Effects of urban infrastructure on aquatic invertebrate diversity, Urban Ecosystems, 23, 4, pp. 831-840, (2020)
  • [3] Yang YF, Wang Q, Chen IF, Et al., Research advance in estuarine zooplankton ecology, Acta Ecalogica Sinica, 26, 2, pp. 72-75, (2006)
  • [4] de Necker L, Brendonck L, van Vuren J, Et al., Aquatic invertebrate community resilience and recovery in response to a Supra-Seasonal drought in an ecologically important naturally Saline Lake, Water, 13, 7, (2021)
  • [5] Wilhelm S, Knudsen, Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea, journal of Experimental Marine Biology and Ecology, 510, pp. 31-45, (2019)
  • [6] Qin CX, Zuo T, Yu G, Et al., Advances in research of environmental DNA (eDNA) in biomass assessment of aquatic ecosvstems, South Chi-na Fisheries Science, 16, 5, pp. 123-128, (2020)
  • [7] Reinholdt JM, Egelyng E, Agersnap S, Et al., Seasonal turnover in community composition of stream-associated macroinvertebrates inferred from freshwater environmental DNA metabarcoding, Environmental DNA, 3, 4, pp. 861-876, (2021)
  • [8] Bush A, Compson ZG, Monk WA, Et al., Studying ecosystems with DNA metabarcoding
  • [9] Lessons from biomonitoring of aquatic macroinverte-brates, Frontiers in Ecology and Evolution, 7, (2019)
  • [10] Brantschen J, Blackman B_C, Walser JC, Et al., Environmental DNA gives comparable results to morphology-based indices of macroinverte-brates in a large-scale ecological assessment, PLoS One, 16, 9, (2021)
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