Modulation of antioxidant enzyme by light and heavy rare earth metals: A case study with catalase

被引：0

作者：

Samal, Rashmi R. ^{[1
,2
]}

Subudhi, Umakanta ^{[1
,2
]}

机构：

[1] Biochemistry & Biophysics Laboratory, Environment & Sustainability Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar

[2] Academy of Scientific and Innovative Research (AcSIR), Ghaziabad

来源：

International Journal of Biological Macromolecules | 2024年 / 283卷

关键词：

Catalase; Enzyme kinetics; Gadolinium; Lanthanides; Metal; Praseodymium; Rare earth elements;

D O I：

10.1016/j.ijbiomac.2024.137820

中图分类号：

学科分类号：

摘要：

The present study highlights the hazardous effect of heavy and light rare earth elements (REEs) on bovine liver catalase (BLC) using a combination of spectroscopic and computational methods. The presence of Praseodymium chloride (PrCl3) and Gadolinium chloride (GdCl3) resulted in a substantial reduction in catalytic efficiency of BLC by approximately 1.8 and 2.6 fold, respectively. The compromised activity was further accompanied by conformational rearrangements at the secondary and tertiary levels as evidenced by circular dichroism (CD) and fluorescence spectroscopy. These analyses revealed a significant decrease in α-helical content and a simultaneous increase in random coils, disrupting intramolecular hydrogen bonding. Furthermore, the zeta potential (ζ) of BLC demonstrated a reversal from negative to positive ζ values upon the addition of PrCl3 and GdCl3, indicating BLC-lanthanide complex formation. Isothermal titration calorimetry (ITC) supports spontaneous interaction with negative free energy favouring endothermic reaction. This was further supported by docking studies which revealed the binding of PrCl3 and GdCl3 within the active site of BLC thus interfering with the catalytic ability to degrade hydrogen peroxide (H2O2). Nevertheless, a significant decline in the melting temperature (Tm) of BLC was observed in the presence of lanthanides suggesting the thermal instability of the enzyme. Thus, a similar approach could be applied to evaluate the hazardous effects of lanthanides on structural and functional changes in other proteins or similar biomolecules. © 2024 Elsevier B.V.

引用

共 68 条

[1]

Zhou B., Li Z., Chen C., Global potential of rare earth resources and rare earth demand from clean technologies, Minerals, 7, (2017)

[2]

Cheisson T., Schelter E.J., Rare earth elements: Mendeleev's bane, modern marvels, Science, 363, pp. 489-493, (2019)

[3]

Evans C.H., Episodes From the History of the Rare Earth Elements (Chemists and Chemistry), Kulwer Academic Publishers, 15, (1996)

[4]

Bhanjadeo M.M., Nayak A.K., Subudhi U., Cerium chloride stimulated controlled conversion of B-to-Z-DNA in self-assembled nanostructures, Biochem. Biophys. Res. Commun., 482, pp. 916-992, (2017)

[5]

Bhanjadeo M.M., Nayak A.K., Subudhi U., Surface-assisted DNA self-assembly: an enzyme-free strategy towards formation of branched DNA lattice, Biochem. Biophys. Res. Commun., 485, pp. 492-498, (2017)

[6]

Bhanjadeo M.M., Baral B., Subudhi U., Sequence-specific B-to-Z transition in self-assembled DNA: a biophysical and thermodynamic study, Int. J. Biol. Macromol., 137, pp. 337-345, (2019)

[7]

Bhanjadeo M.M., Nial P.S., Sathyaseelan C., Singh A.K., Rathinavelan T., Subudhi U., Biophysical interaction between lanthanum chloride and (CG)n or (GC)n repeats: a reversible B-to-Z DNA transition, Int. J. Biol. Macromol., 216, pp. 698-709, (2022)

[8]

Nayak A.K., Mishra A., Jena B.S., Mishra B.K., Subudhi U., Lanthanum induced B-to-Z transition in self-assembled Y-shaped branched DNA structure, Nat. Sci. Rep., 6, pp. 26855-26867, (2016)

[9]

Nayak A.K., Rath S.K., Subudhi U., Preparation of stable branched DNA nanostructures: process of cooperative self-assembly, J. Phys. Chem. B, 123, pp. 3591-3597, (2019)

[10]

Nial P.S., Subudhi U., Zeta potential of Z-DNA: a new signature to study B-Z transition in linear and branched DNA, Int. J. Biol. Macromol., 266, (2024)

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