Transcriptome Analysis of Two Flaxseed Cultivars Grown in Xinjiang and Mining of Differentially Expressed Genes Related to Flaxseed Oil Aroma

被引：1
作者：
Yang J. [1 ]
Li H. [1 ]
Lin Y. [1 ]
Liu W. [1 ]
Wei C. [1 ]
机构：
[1] Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Food Science and Technology, Shihezi University, Shihezi
来源：
Shipin Kexue/Food Science | 2022年 / 43卷 / 02期
关键词：
Differentially expressed genes; Flaxseed; Functional annotation; Transcriptome;
D O I：
10.7506/spkx1002-6630-20201120-213
中图分类号：
学科分类号：
摘要：
This study was executed in order to enrich the transcriptome information of flaxseeds and explore the reasons for the difference in the aroma of flaxseed oils from different cultivars at the molecular level. Two specialty flaxseed cultivars(Yiya-3 and Tianya-6) in Xinjiang were selected to perform transcriptome sequencing analysis using the Illumina highthroughput sequencing platform and a variety of bioinformatic methods. The results showed that a total of 61 995 756 highquality clean reads were obtained. Further, a total of 33 218 unigenes were obtained after de novo assembling of the sequence data. Non-Redundant Protein (NR) database annotations revealed that the flaxseed transcriptome had the highest sequence similarity to Jatropha curcas, and 17 269 unigenes were annotated in the Cluster of Orthologous Group (COG) database, which were divided into 22 branches in three categories, including cellular processes and signals, information storage and processing, and metabolism. There were 1 658 significant differentially expressed genes (DEGs) identified between the two cultivars. Compared to Tianya-6, 825 genes were up-regulated and 833 genes were down-regulated in Yiya-3.The annotation of the DEGs in the Gene Ontology (GO) database showed that they were distributed in three categories:biological processes, cellular components and molecular functions. The 380 DEGs annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database were mainly enriched in fatty acid metabolism and other metabolic pathways, and 17 DEGs could be annotated in the fatty acid metabolism pathway, with eight being annotated in the unsaturated fatty acid metabolism pathway. This study provides a theoretical basis and technical support for the analysis of the difference in the aroma of flaxseed oils from different cultivars in Xinjiang. © 2022, China Food Publishing Company. All right reserved.
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页码：70 / 76
页数：6
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