Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

被引：0

作者：

Gu Y. ^{[1
]}

Tang H. ^{[1
]}

Li S. ^{[2
]}

Ma L. ^{[1
]}

Wang K. ^{[1
,2
]}

Yang L. ^{[1
]}

机构：

[1] College of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang

[2] Shijiazhuang Technology Innovation Center, Chemical Poison Detection and Risk Early Warning, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 18期

关键词：

fruit products; meat sauce; QuEChERS; tetracycline antibiotics; ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry;

D O I：

10.13386/j.issn1002-0306.2022100072

中图分类号：

学科分类号：

摘要：

A method for the determination of 10 tetracycline antibiotics in fruit products and meat sauce was established by QuEChERS coupled with ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS). The optimal experimental conditions were determined by optimizing the mass spectrometric parameters, chromatographic conditions and pre-treatment techniques. The samples were extracted by 0.2% formic acid acetonitrile under ultrasonication, and then purified with PSA for fruit products and C18 for meat sauce. The target analytes were; separated on a ZORBAX SB-Aq column (2.1 mm×150 mm, 3.5 μm), and detected by UPLC-MS/MS. The results demonstrated that the 10 tetracycline antibiotics showed good linearity in the respective linear range with the correlation coefficients (r) higher than 0.999. The limits of detection (S/N=3) were 0.09~3.92 μg/kg, and the limits of quantification (S/N=10) were 0.31~13.05 μg/kg. The average recoveries at three different spiked levels of 75, 300 and 750 μg/kg ranged from 70.0% to 101.0% with the relative standard deviation (RSD) of 1.0%~10.3% (n=7). The method is sensitive, simple and suitable for the rapid and simultaneous detection of 10 tetracycline antibiotics in fruit products and meat sauce. © 2023 Editorial Department of Science and Technology of Food Science. All rights reserved.

引用

页码：313 / 320

页数：7

共 33 条

[1]

YU J H, LIU H, WANG Y T, Et al., Fluorescent sensing system based on molecularly imprinted phase-change microcapsules and carbon quantum dots for high-efficient detection of tetracycline [J], Journal of Colloid and Interface Science, 599, pp. 332-350, (2021)

[2]

ZHAO C X., Preparation of functionalized magnetic polymer microspheres for magnetic dispersion extraction of tetracyclines from milk samples, (2013)

[3]

MANUEL C C, MARIA J F S, GUSTAVO F C, Et al., Competitive adsorption and desorption of three tetracycline antibiotics on bio-sorbent materials in binary systems[J], Environmental Research, 190, (2020)

[4]

TAN Y, LIU Q Z., Occurrence and control of sweet cherry bush disease[J], Nongcun Baishitong, 16, (2018)

[5]

CHEN G Q, LU L M, DU D C, Et al., Research progress in prevention and control of citrus huanglong disease[J], Zhejiang Ganju, 29, 3, (2012)

[6]

WU Y H., Study on immunolateral chromatography detection method of tetracycline and penicillin residues in milk, (2020)

[7]

BU X N., Studies on confirmation analysis of five tetracyclines and its three metabolites in chicken muscle via HPLCMS/MS, (2020)

[8]

ZHANG L N, WANG Y L, JIA L, Et al., Ultrasensitive and visual detection of tetracycline based on dual-recognition units constructed multicolor fluorescent nano-probe[J], Journal of Hazardous Materials, 409, (2021)

[9]

LI Y, WANG Y, DU P Y, Et al., Fabrication of carbon dots@hierarchical mesoporous ZIF-8 for simultaneous ratiometric fluorescence detection and removal of tetracycline antibiotics[J], Sensors and Actuators:B. Chemical, 358, (2022)

[10]

WANG W, DENG P Y, LIU X Q, Et al., A CsPbBr<sub>3</sub> quantum dots/ultra-thin BN fluorescence sensor for stability and highly sensitive detection of tetracycline[J], Microchemical Journal, 162, (2021)

← 1 2 3 4 →