Rapid and sensitive detection of domoic acid in shellfish using a magnetic bead-based competitive ELISA with a high-affinity peptide as a molecular binder

被引:1
作者
Kim, Ji Hong [1 ]
Cho, Chae Hwan [1 ]
Park, Tae Jung [2 ]
Park, Jong Pil [1 ]
机构
[1] Department of Food Science and Technology, and GreenTech-Based Food Safety Research Group, BK21 Four, Chung-Ang University, Anseong
[2] Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul
关键词
Affinity peptide; dc-ELISA; Domoic acid; Magnetic beads; Marine toxin;
D O I
10.1016/j.chemosphere.2024.143274
中图分类号
学科分类号
摘要
Addressing the critical health concerns posed by domoic acid (DA), a neurotoxic compound produced by toxic marine algae and bioaccumulated in shellfish, necessitates the development of a rapid, precise, and robust detection system. Traditional DA detection methods have stability and sensitivity issues, which hinder effective toxin detection. To overcome these limitations, we developed a novel direct competitive enzyme-linked immunosorbent assay (dc-ELISA) platform that utilizes peptide-immobilized magnetic beads (MGBs/peptide). The affinity peptides identified through phage display and chemically synthesized with biotin labels present an innovative alternative to conventional antibodies for ELISA applications. Streptavidin-modified MGBs were used as the bioreceptor carriers to facilitate magnetic separation and simplify sample preparation, making the MGB/peptide-based dc-ELISA platform an ideal tool for comprehensive monitoring efforts. The developed platform exhibits a detection range of 0.5–10 ng mL−1 and a low limit of detection of 0.29 ng mL−1, offering enhanced sensitivity and cost-effectiveness. Moreover, our developed dc-ELISA demonstrated a high recovery rate when validated with DA-spiked CRM-mussel samples. This method overcomes the limitations of traditional detection techniques and offers a scalable and efficient approach to marine toxin surveillance with improved marine environmental monitoring and public health management. © 2024 Elsevier Ltd
引用
收藏
相关论文
共 5 条
[1]   Development of a rapid magnetic bead-based immunoassay for sensitive detection of zearalenone [J].
Zhao, Fengchun ;
Shen, Qiang ;
Wang, Huimin ;
Han, Xiao ;
Yang, Zhengyou .
FOOD CONTROL, 2017, 79 :227-233
[2]   High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder [J].
Hwang, Hye Jin ;
Ryu, Myung Yi ;
Park, Chan Young ;
Ahn, Junki ;
Park, Hyun Gyu ;
Choi, Changsun ;
Ha, Sang-Do ;
Park, Tae Jung ;
Park, Jong Pil .
BIOSENSORS & BIOELECTRONICS, 2017, 87 :164-170
[3]   Magnetic bead-based chemiluminescence detection of sequence-specific DNA by using catalytic nucleic acid labels [J].
Cao, ZJ ;
Li, ZX ;
Zhao, YJ ;
Song, YM ;
Lu, JZ .
ANALYTICA CHIMICA ACTA, 2006, 557 (1-2) :152-158
[4]   Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples [J].
Qianqian Jiang ;
Yang Li ;
Lin Huang ;
Jinling Guo ;
Ailin Wang ;
Cuiping Ma ;
Chao Shi .
Analytical and Bioanalytical Chemistry, 2023, 415 :427-438
[5]   Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples [J].
Jiang, Qianqian ;
Li, Yang ;
Huang, Lin ;
Guo, Jinling ;
Wang, Ailin ;
Ma, Cuiping ;
Shi, Chao .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2023, 415 (03) :427-438