Isolation and Identification of the Plant Endophyte R-13 and Its Effect on Cadmium Accumulation in Solanum nigrum L.

被引：2

作者：

Pang J. ^{[1
,2
]}

Liu Y.-M. ^{[1
]}

Huang Y.-C. ^{[2
]}

Wang C.-R. ^{[2
]}

Liu B. ^{[3
]}

Liu Z.-Q. ^{[2
]}

Huang Y.-Z. ^{[2
]}

Huang Y.-F. ^{[3
]}

Zhang C.-B. ^{[2
]}

机构：

[1] School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin

[2] Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin

[3] Agricultural Resources and Environment Institute, Guangxi Academy of Agricultural Sciences, Nanning

来源：

Huanjing Kexue/Environmental Science | 2021年 / 42卷 / 09期

关键词：

Cadmium; Endophytes; Herbaspirillum; Phytoremediation; Solanum nigrum L;

D O I：

10.13227/j.hjkx.202101192

中图分类号：

学科分类号：

摘要：

The combination of endophytes and hyperaccumulator plants can significantly improve the efficiency of heavy metal phytoremediation in contaminated soil. A plant endophyte named Herbaspirillum R-13 was isolated from rice roots in a cadmium (Cd) contaminated paddy field. This strain exhibited a strong tolerance to Cd2+ and could grow on a solid medium with a Cd2+ concentration of 300 mg•kg-1. The R-13 strain was able to produce siderophores and Indole acetic acid (IAA), through color reactions. In addition, Pikovskaya's and Ashby's solid medium tests showed that the R-13 strain had a lower capacity for dissolving phosphorus but a higher capacity for fixing nitrogen. In the pot experiment, high-throughput sequencing technology was used to track the colonization of the R-13 strain in Solanum nigrum L. roots. Three days after inoculation, the relative abundance of Herbaspirillum in the root of Solanum nigrum L. had increased by 201.88% compared to the blank control (CK) and after two inoculations, the relative abundance of Herbaspirillum in the root of Solanum nigrum L. had increased by 1182.44% compared to CK. The relative abundance of Herbaspirillum in the root of Solanum nigrum L. began to decrease significantly from 5 days after inoculation. Inoculation with 20 mL•pot-1 of R-13 fermentation broth resulted in no significant effects on the Cd content of roots, stems, leaves, or fruits of S. nigrum L. With 40 mL•pot-1 of fermentation broth, the Cd content of vegetative organs and fruits was significantly increased. When it reached 200 mL•pot-1, the Cd content of vegetative organs was the highest, with Cd concentrations in the roots, stems, leaves, and fruits increasing by 84.42%, 43.67%, 64.06%, and 20.29%, respectively. In conclusion, root inoculation with endophytic Herbaspirillum R-13 can significantly increase the relative content of Herbaspirillum in the root system and enhance Cd absorption of S. nigrum L. Therefore, this strain has excellent prospects for application in the phytoremediation of soil contaminated with Cd. © 2021, Science Press. All right reserved.

引用

页码：4471 / 4480

页数：9

共 49 条

[1] Liu L H, Yang Z C, Zhao L., Research progress in adsorption materials for heavy metals, Materials China, 37, 2, pp. 100-108, (2018)
[2] Tong S M, Hou W J, Yang Q, Et al., Accumulation characteristic and health risk assessment of soil heavy metals in different functional areas in Liupanshui, Journal of Environment and Health, 36, 3, pp. 214-219, (2019)
[3] Li M Z, He Z S, Gu S Y., Advances on epigenetic regulation in the cadmium toxicity, Modern Preventive Medicine, 47, 1, pp. 119-122, (2020)
[4] Du S, Zhou Y Y, Zhang L., Application of stable isotopes (δ13C and δ15N) in studies on heavy metals bioaccumulation in Daya Bay food web, Chinese Journal of Applied Ecology, 28, 7, pp. 2327-2338, (2017)
[5] Yu J J, Shi H D, Wang M H, Et al., Identification of soil cadmium pollution sources in affected areas of key pollution enterprises in Xiangjiang sub-Basin, Research of Environmental Sciences, 33, 4, pp. 1013-1020, (2020)
[6] Chen H P, Tang Z, Wang P, Et al., Geographical variations of cadmium and arsenic concentrations and arsenic speciation in Chinese rice, Environmental Pollution, 238, pp. 482-490, (2018)
[7] Yang Y M, Chen C X, Lei J R, Et al., Cadmium accumulation and growth of different Sichuan wheat varieties under cadmium stress, Southwest China Journal of Agricultural Sciences, 31, 9, pp. 1796-1801, (2018)
[8] Liu S A, Wu H, Hu J M, Et al., Heavy metal passivation technology for farmland soil health: research and application trend, Journal of Agriculture, 10, 3, pp. 6-11, (2020)
[9] Sumiahadi A, Acar R., A review of phytoremediation technology: heavy metals uptake by plants, IOP Conference Series: Earth and Environmental Science, 142, (2018)
[10] Lin Y Y, Chen H R, Liu B M, Et al., Study on low-cadmium rice mutants induced by 12C6+ ion beam, Nuclear Physics Review, 33, 4, pp. 488-493, (2016)

← 1 2 3 4 5 →