Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation

被引：22

作者：

Alijagic A. ^{[1
]}

Barbero F. ^{[2
]}

Gaglio D. ^{[3
,4
]}

Napodano E. ^{[4
]}

Benada O. ^{[5
]}

Kofroňová O. ^{[5
]}

Puntes V.F. ^{[2
,6
,7
]}

Bastús N.G. ^{[2
]}

Pinsino A. ^{[1
]}

机构：

[1] Consiglio Nazionale delle Ricerche, Istituto per la Ricerca e l'Innovazione Biomedica (IRIB), Palermo

[2] Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, Barcelona

[3] Consiglio Nazionale delle Ricerche, Istituto di Bioimmagini e Fisiologia Molecolare (IBFM), Segrate, MI

[4] SYSBIO.IT, Centre of Systems Biology, University of Milano-Bicocca, Milano

[5] Institute of Microbiology of the Czech Academy of Sciences, Prague

[6] Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona

[7] Vall d Hebron, Institut de Recerca (VHIR), Barcelona

来源：

Journal of Hazardous Materials | 2022年 / 402卷

基金：

欧盟地平线“2020”;

关键词：

Immune metabolic rewiring; Immunoreactivity; Innate defence response; Nano-recognition; Sea urchin immune cells;

D O I：

10.1016/j.jhazmat.2020.123793

中图分类号：

学科分类号：

摘要：

We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP–AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP–AuNPs (1 and 10 μg mL−1), we applied an extensive nano-scale approach, including particle physicochemical characterisation involving immunology, cellular biology, and metabolomics. The interaction between PVP–AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein “corona” surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP–AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP–AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP–AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP–AuNPs and provides more details regarding the immunogenicity of these NPs. © 2020 Elsevier B.V.

引用

共 74 条

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