Tyrosinase Inhibitory Peptide from the Hydrolysates of Eel Bone: Optimization of Preparation, Inhibition Kinetics and Molecular Docking

被引：1
|
作者：
Bai, Xiaolin [1 ]
Sun, Yejing [1 ]
Zhang, Yuhang [1 ]
Fang, Yizhou [1 ]
Jiang, Han [1 ]
Huang, Guangrong [1 ]
机构：
[1] China Jiliang Univ, Coll Life Sci, Zhejiang Prov Key Lab Special Agr Qual & Hazard Co, Xueyuan St 258, Hangzhou 310018, Zhejiang, Peoples R China
来源：
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY | 2024年 / 33卷 / 08期
关键词：
Eel bone; tyrosinase inhibitory peptide; Optimization; inhibition kinetics; molecular docking; RICE BRAN PROTEIN; MECHANISM; AGENTS;
D O I：
10.1080/10498850.2024.2415447
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The main purpose of this study was to discover new tyrosinase inhibitory peptides from eel bone hydrolysates. After enzyme screening, alkaline protease was utilized, and optimal conditions were achieved through response surface optimization: solid-liquid ratio (1:25), time (5 h), and enzymolysis base ratio (3500 U/g). Inhibitory peptides of 1-3 kDa were isolated (IC50 = 2.162 mg/mL), and the inhibition was determined to be non-competitive and reversible. Subsequent LC-MS/MS and molecular docking revealed that the peptide with the lowest binding energy exhibited hydrogen bonding, hydrophobic interactions, and provided a potential explanation for the mechanism of inhibition by the peptides.
引用
收藏
页码：637 / 649
页数：13
相关论文
共 50 条
  • [21] Inhibitory effects of four anthraquinones on tyrosinase activity: Insight from spectroscopic analysis and molecular docking
    Zeng, Hua-jin
    Sun, Dong-qin
    Chu, San-hui
    Zhang, Jun-jun
    Hu, Gui-zhou
    Yang, Ran
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 160 : 153 - 163
  • [22] Optimization of Enzymatic Preparation of ACE Inhibitory Peptide from Sheep Milk Casein and Its Inhibition Mechanism
    Tang H.
    Zhang Y.
    Ge W.
    Song Y.
    Wang H.
    Wang S.
    Journal of Chinese Institute of Food Science and Technology, 2022, 22 (06) : 220 - 231
  • [23] The inhibitory effect of pyrogallol on tyrosinase activity and structure: Integration study of inhibition kinetics with molecular dynamics simulation
    Xiong, Shang-Ling
    Lim, Gyu Tae
    Yin, Shang-Jun
    Lee, Jinhyuk
    Si, Yue-Xiu
    Yang, Jun-Mo
    Park, Yong-Doo
    Qian, Guo-Ying
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 121 : 463 - 471
  • [24] Identification and Molecular Docking Study of a Novel Angiotensin-I Converting Enzyme Inhibitory Peptide Derived from Enzymatic Hydrolysates of Cyclina sinensis
    Yu, Fangmiao
    Zhang, Zhuangwei
    Luo, Liwang
    Zhu, Junxiang
    Huang, Fangfang
    Yang, Zuisu
    Tang, Yunping
    Ding, Guofang
    MARINE DRUGS, 2018, 16 (11):
  • [25] Screening of Xanthine Oxidase Inhibitory Peptide from Bass Myosin by Molecular Docking
    Zhao, Guiqin
    Li, Tingting
    Song, Minjie
    Sun, Hong
    Li, Jianrong
    Xie, Jing
    Deng, Shanggui
    Journal of Chinese Institute of Food Science and Technology, 2021, 21 (06): : 81 - 91
  • [26] Preparation, identification, and molecular docking of novel elastase inhibitory peptide from walnut (Juglans regia L.) meal
    Xiong, Yu
    Peng, Peng
    Chen, Shi-Jia
    Chang, Min
    Wang, Qian
    Yin, Sheng-Nan
    Ren, Di-Feng
    FOOD CHEMISTRY: MOLECULAR SCIENCES, 2022, 5
  • [27] Molecular docking and antihypertensive effects of a novel angiotensin-I converting enzyme inhibitory peptide from yak bone
    Gao, Xinchang
    Bu, Fan
    Yi, Dalong
    Liu, Huaigao
    Hou, Zhiying
    Zhang, Chaoying
    Wang, Chang
    Lin, Jin-Ming
    Dang, Yali
    Zhao, Yufen
    FRONTIERS IN NUTRITION, 2022, 9
  • [28] Novel ACE inhibitory peptides derived from whey protein hydrolysates: Identification and molecular docking analysis
    Li, Xiaoyi
    Feng, Chunsong
    Hong, Hui
    Zhang, Yan
    Luo, Zhigang
    Wang, Quanyu
    Luo, Yongkang
    Tan, Yuqing
    FOOD BIOSCIENCE, 2022, 48
  • [29] Tyrosinase Inhibitory Kinetics, LC-QTOF-MS Based Chemical Profiling and Molecular Docking of Phytochemicals from Dillenia indica L. Barks
    Gogoi, Pinku
    Kumar, Amit
    Das, Aparoop
    Baishya, Gakul
    CHEMISTRY AFRICA-A JOURNAL OF THE TUNISIAN CHEMICAL SOCIETY, 2023, 6 (04): : 1799 - 1810
  • [30] Tyrosinase Inhibitory Kinetics, LC-QTOF-MS Based Chemical Profiling and Molecular Docking of Phytochemicals from Dillenia indica L. Barks
    Pinku Gogoi
    Amit Kumar
    Aparoop Das
    Gakul Baishya
    Chemistry Africa, 2023, 6 : 1799 - 1810
12345