Effect Mechanism of Lignin Isolated from Diverse Types Woods on Cellulase Adsorption

被引：0
作者：
Jiang, Kangjie [1 ]
Wu, Wenjuan [1 ]
Huang, Lijing [1 ]
Li, Jiaquan [1 ]
Li, Kongyan [1 ]
机构：
[1] Jiangsu Key Laboratory of Pulp and Paper Science and Technology Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing
来源：
Linye Kexue/Scientia Silvae Sinicae | 2024年 / 60卷 / 07期
关键词：
enzymatic adsorption; enzymatic hydrolysis; lignin; QCM-D; structural unit;
D O I：
10.11707/j.1001-7488.LYKX20220547
中图分类号：
学科分类号：
摘要：
【Objective】In order to explore the effect of lignin on enzyme adsorption in substrate enzyme hydrolysis, using the proto lignin as material, isolated from different species of poplar, eucalyptus and acacia wood, and the adsorption behavior of cellulase on lignin films was studied by quartz crystal microbalance with dissipation (QCM-D) technology.【 Method】 The structures of isolated lignins were characterized by 2D NMR. The lignin/cellulose biosensor was prepared by spin coating method and characterized by the surface morphology before and after enzyme adsorption, and the QCM-D technology was used to explore the influence of lignin with different structural unit on cellulase adsorption in situ and in real time.【Result】Lignins from poplar, eucalyptus and acacia were GS lignin, and the main connection mode between structural units was β-O-4, and their S/G ratios were 1.09, 1.73 and 1.22, respectively. After the injection of the cellulase, the cellulase was evenly distributed on the surface of the lignin film, and they were bound tightly. The roughness of the lignin film of eucalyptus was higher than that of poplar and acacia at the end of injection. By QCM-D analysis, the poplar lignin film had minimal capacity to adsorb cellulase while eucalyptus lignin largest capacity.【 Conclusion】 Different structural unit types in lignin structure also show different adsorption capacity to cellulase. The enzymatic hydrolysis process of composite film can be divided into four stages: adsorption, rapid hydrolysis, slow hydrolysis and hydrolysis equilibrium. Combined with the kinetic parameters of enzymatic hydrolysis, eucalyptus lignin with higher S/G ratio takes longer time to reach the maximum enzymatic hydrolysis conversion and lower hydrolysis degree. © 2024 Chinese Society of Forestry. All rights reserved.
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页码：140 / 148
页数：8
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