Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form)

被引：0
|
作者：
Kokoulin, Maxim S. [1 ]
Belova, Vlada S. [1 ,2 ]
Romanenko, Lyudmila A. [1 ]
机构：
[1] Russian Acad Sci, GB Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia
[2] Far Eastern Fed Univ, 10 Ajax Bay,Russky Isl, Vladivostok 690922, Russia
来源：
CARBOHYDRATE RESEARCH | 2024年 / 545卷
关键词：
Marine bacterium; Bacterial dissociation; Pseudoalteromonas agarivorans; Lipopolysaccharide; O; -polysaccharide; NMR; Structure; CONFIGURATION;
D O I：
10.1016/j.carres.2024.109300
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D 1H and 13C NMR spectroscopy, including 1H,1H COSY, 1H,1H TOCSY, 1H,1H ROESY and 1H,13C HSQC, and 1H,13C HMBC experiments. The following new structure of the OPS from P. agarivorans KMM 232 (O-form) containing 2-acetamido-2-deoxyD-glucose (D-GlcNAc), D-glucose (D-Glc), D-glucuronic acid (D-GlcA), 4,6-O-[(R)-1-carboxyethylidene]-D-galactose [D-Galp4,6 (R-Pyr)] and two residues of D-galactose (D-Gal) was established:
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