Selenium attenuated food borne cadmium-induced intestinal inflammation in red swamp crayfish (Procambarus clarkii) via regulating PI3K/Akt/NF-κB pathway

被引：18
作者：
Yang H. [1 ]
Mo A. [1 ]
Yi L. [1 ]
Wang J. [1 ]
He X. [1 ]
Yuan Y. [1 ,2 ,3 ]
机构：
[1] College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei, Wuhan
[2] Shuangshui Shuanglu Institute, Huazhong Agricultural University, Wuhan
[3] National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan
来源：
Chemosphere | 2024年 / 349卷
基金：
中国国家自然科学基金;
关键词：
Cadmium; Gut inflammation; PI3K/Akt/NF-κB pathway; Procambarus clarkii; Selenium;
D O I：
10.1016/j.chemosphere.2023.140814
中图分类号：
学科分类号：
摘要：
Selenium (Se), an indispensable micronutrient for living organisms, has been extensively studied for its heavy metal-detoxifying properties in diverse biological systems and tissues. Nevertheless, it is not entirely certain whether Se can effectively protect against Cadmium (Cd)-induced gut inflammation, especially in aquatic animals. In this study, we employed various approaches, including transcriptome profiling, histological examinations, assessment of antioxidant enzyme activities, and analysis of gut microbiota composition to investigate the effects on crayfish growth and intestinal health after exposure to dietary Cd (15 mg kg−1 diet) and Se (15 mg kg−1 diet) individually or in combination for 8 weeks. The results revealed that dietary Cd exposure resulted in reduced body weight and survival rates, along with an increased occurrence of intestinal inflammation. Nevertheless, Se supplementation proved effective in mitigating the adverse effects of Cd on growth and gut health. Se exhibited a remarkable ability to counteract the disruption of gut antioxidant abilities induced by dietary Cd, as evidenced by the observed increases in ROS and MDA contents, decrease in GSH levels, and inhibition of antioxidative enzyme activities. At the concentration of 6 mg kg−1 in the diet, Se was found beneficial for maintaining gut microbiota richness and diversity. Among them, Flavobacterium, Thermomonas, and Chloronema displayed a weak negative correlation with the rate of gut inflammation. Meanwhile, the levels of short chain fatty acids (SCFAs), including acetic acid (AA) and butanoic acid (BA), showed a significant increase in the Se–Cd group compared to the Cd-only group. Furthermore, transcriptome analysis exhibited significant responses of the PI3K/Akt and NF-κB pathways following crayfish exposure to dietary Se and Cd, either separately or in combination. In short, this study provides a new evidence regarding the molecular mechanisms through which Se could regulate the PI3K/Akt and NF-κB pathways, either directly or indirectly via ROS and SCFAs, thereby alleviating Cd-induced gut inflammation in crayfish. © 2023
引用
收藏
相关论文
共 84 条
[1]  
Abdel-Tawwab M., Wafeek M., Response of Nile tilapia, Oreochromis niloticus (L.) to environmental cadmium toxicity during organic selenium supplementation, J. World Aquacult. Soc., 41, 1, pp. 106-114, (2010)
[2]  
Abigail P., Moreno R., Medesani D.A., Rodriguez E.M., Inhibition of molting by cadmium in the crab Chasmagnathus granulata (Decapoda Brachyura, Aquat. Toxicol., 64, pp. 155-164, (2003)
[3]  
Amato K.R., Yeoman C.J., Kent A., Righini N., Carbonero F., Estrada A., Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes, ISME J., 7, 7, pp. 1344-1353, (2013)
[4]  
Andersen O., Nielsen J.B., Svendsen P., Oral cadmium chloride intoxication in mice: diethyldithiocarbamate enhances rather than alleviates acute toxicity, Toxicology, 52, pp. 331-342, (1988)
[5]  
Ankley P.J., Graves S.D., Xie Y., DeBofsky A., Weber A., Brinkmann M., Palace V.P., Liber K., Hecker M., Janz D.M., Giesy J.P., Effects of in situ experimental selenium exposure on finescale dace (Phoxinus neogaeus) gut microbiome, Environ. Res., 212, (2022)
[6]  
Baillieul M., Blust R., Analysis of the swimming velocity of cadmium-stressed Daphnia magna, Aquat. Toxicol., 44, pp. 245-254, (1999)
[7]  
Barata C., Markich S.J., Baird D.J., Soares A.M., The relative importance of water and food as cadmium sources to Daphnia magna Straus, Aquat. Toxicol., 61, 3-4, pp. 143-154, (2002)
[8]  
Benvenga S., Marini H.R., Micali A., Freni J., Pallio G., Irrera N., Squadrito F., Altavilla D., Puzzolo D., Minutoli L., Protective effects of myo-inositol and selenium on cadmium-induced thyroid toxicity in mice, Nutrients, 12, 5, (2020)
[9]  
Chen M.H., Li X.J., Fan R.F., Cao C.Y., Yao H.D., Xu S.W., Selenium antagonizes cadmium-induced apoptosis in chicken spleen but not involving Nrf2-regulated antioxidant response, Ecotoxicol. Environ. Saf., 145, pp. 503-510, (2017)
[10]  
Chen X.M., Bi M.Y., Yang J., Cai J.Z., Zhang H.R., Zhu Y., Zheng Y.Y., Liu Q., Shi G.L., Zhang Z.W., Cadmium exposure triggers oxidative stress, necroptosis, Th1/Th2 imbalance and promotes inflammation through the TNF-α/NF-κB pathway in swine small intestine, J. Hazard Mater., 421, (2022)
123456789