Facile preparation of titanium functionalized cross-linked chitosan polymer for phosphoproteome analysis in serum

被引:0
|
作者
Sheng, Xiuqin [1 ]
Guo, Yimin [1 ]
Ding, Chuan-Fan [1 ]
Yan, Yinghua [1 ]
机构
[1] Ningbo Univ, Inst Mass Spectrometry, Sch Mat Sci & Chem Engn, Key Lab Adv Mass Spectrometry & Mol Anal Zhejiang, Ningbo 315211, Zhejiang, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2024年 / 1247卷
关键词
MALDI-TOF MS; Phosphopeptides; Immobilized metal affinity chromatography; HIGHLY SELECTIVE ENRICHMENT; PHOSPHORYLATION;
D O I
10.1016/j.jchromb.2024.124347
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Efficient phosphopeptide enrichment is extremely important for proteomics research. In this work, chitosan (CTs), 2,3-dihydroxyterephthalaldehyde (2,3-DHA), and carbohydrazide (CHZ) are polymerized to generate the polymer (DHA-CTs-CHZ), and then the polymer (DHA-CTs-CHZ) is coupled with a significant number of titanium ions to enrich phosphopeptides. The material exhibits high selectivity (5000:1 M ratio of BSA to beta-casein), sensitivity (0.001 fmol/mu L), loading (83.3 mu g/mg), recovery (98.6 +/- 1.2 %), and effective size exclusion for phosphopeptide enrichment. In addition, 46 phosphopeptides and 31 phosphorylated sites associated with 27 phosphorylated proteins were successfully captured from the serum of normal subjects, while 47 phosphopeptides and 35 phosphorylated sites associated with 30 phosphorylated proteins were successfully captured from Alzheimer's disease (AD) patients' serum.
引用
收藏
页数:6
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