Effects of Simulated Gastrointestinal Digestion on Active Components and Antioxidant Activity of Fermented Tea (Camellia sinensis) Jiaosu in Vitro

被引:0
|
作者
Ye C. [1 ]
Fan H. [1 ]
Bao H. [1 ]
Wang Z. [1 ]
Sha R. [1 ]
Fang S. [2 ]
Hu X. [3 ]
Jiang L. [3 ]
Li D. [3 ]
Yuan M. [3 ]
Mao J. [1 ,4 ]
机构
[1] Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, School of Biological and Chemical Engineering, Zhejiang Un
[2] Shaoxing University Yuanpei College, Shaoxing
[3] Jinhua Academy of Agricultural Sciences, Jinhua
[4] Zhejiang Industry Polytechnic College, Shaoxing
关键词
antioxidant activity; fermented tea Jiaosu; flavonoids; in vitro simulated gastrointestinal digestion; polyphenols; procyanidins;
D O I
10.13386/j.issn1002-0306.2021010099
中图分类号
学科分类号
摘要
In order to explore the changes of active components and antioxidant activities of ancient fermented tea (Camellia sinensis) Jiaosu after simulated gastric and intestinal digestion, the contents of total phenols, flavones and procyanidins in ancient fermented tea (Camellia sinensis) Jiaosu were determined by the method of in vitro simulated gastric and intestinal digestion. Meanwhile, the changes in the contents of five catechins in tea Jiaosu was investigated before and after gastric and intestinal digestion, and the changes of antioxidant activity in vitro were further studied. The results showed that the contents of total phenols, total flavonoids, ABTS cationic free radical scavenging ability and reducing power were significantly increased (P<0.05), while the content of procyanidins and DPPH free radical scavenging ability had no significant change (P>0.05). In the simulated intestinal digestion, the total phenol content and ABTS cationic free radical scavenging ability increased significantly (P<0.05), while the total flavone content, DPPH free radical scavenging ability and reducing power had no significant change (P>0.05), while the procyanidin content decreased significantly (P<0.05). The contents of catechins, epigallocatechin, epicatechin and epicatechin gallate decreased by 74.58%, 64.40%, 86.95% and 48.21%, respectively. These results indicated that pepsin and gastric acid could promote the release of antioxidant active substances in the simulated gastrointestinal digestion process of ancient tea leaf enzyme, which had good antioxidant activity and was expected to be applied in the development of antioxidant products. © 2021 Editorial Department of Science and Technology of Food Science. All rights reserved.
引用
收藏
页码:64 / 72
页数:8
相关论文
共 32 条
  • [21] Arnao M B, Cano A, Acosta M., The hydrophilic and lipophilic contribution to total antioxidant activity[J], Food Chemistry, 73, 2, pp. 239-244, (2001)
  • [22] Tohidi B, Rahimmalek M, Arzani A., Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of thymus species collected from different regions of Iran[J], Food Chemistry, 220, pp. 153-161, (2017)
  • [23] Li Ru, Zhu Yi, Effect of simulated gastrointestinal digestion in vitro on active substances, antioxidant function and differential metabolites of radish sprouts, Journal of Chinese Institute of Food Science and Technology, 19, 4, pp. 61-71, (2019)
  • [24] Li Shu, Wang Xieyi, Zhai Yuxin, Et al., Stability of polyphenols during in vitro digestion, Food Science, 37, 13, pp. 1-5, (2016)
  • [25] Tan Heping, Zou Yan, Ye Shanrong, Et al., Review of tea polyphenols analyses for tea, China Measurement & Testing Technology, 4, pp. 4-11, (2008)
  • [26] Record I R, Lane J M., Simulated intestinal digestion of green and black teas[J], Food Chemistry, 73, 4, pp. 481-486, (2001)
  • [27] Tenore G C, Campiglia P, Giannetti D, Et al., Simulated gastrointestinal digestion, intestinal permeation and plasma protein interaction of white, green, and black tea polyphenols[J], Food Chemistry, 169, 15, pp. 320-326, (2015)
  • [28] Green R J, Murphy A S, Schulz B, Et al., Common tea formulations modulate in vitro digestive recovery of green tea catechins[J], Molecular Nutrition & Food Research, 51, 9, pp. 1152-1162, (2007)
  • [29] Mochizuki M, Yamazaki S I, Kano K, Et al., Kinetic analysis and mechanistic aspects of autoxidation of catechins[J], Biochim Biophys Acta, 1569, 1-3, pp. 35-44, (2002)
  • [30] Ge Yanlin, Ultrasonic assisted extraction technology and its antioxidant activity of Vitis amurensis Rupr skin anthocyanin, Food Research and Development, 39, 10, pp. 48-55, (2018)