Exploring phosphate impact on arsenate uptake and distribution in freshwater phytoplankton: Insights from single-cell ICP-MS

被引：0

作者：

Alam, Md Shah ^{[1
]}

Wong, Kuo H. ^{[2
]}

Ishikawa, Akari ^{[1
]}

Li, Meng ^{[1
]}

Zai, Yinghan ^{[1
]}

Papry, Rimana Islam ^{[3
]}

Mashio, Asami S. ^{[2
]}

Rahman, Ismail M.M. ^{[4
]}

Hasegawa, Hiroshi ^{[2
]}

机构：

[1] Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa

[2] Institute of Science and Engineering, Kanazawa University, Kakuma, Kanazawa

[3] Department of Environmental Science, College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, 4 Embankment Road, Sector 10, Uttara, Dhaka

[4] Institute of Environmental Radioactivity, Fukushima University, 1 Kanayagawa, Fukushima City, Fukushima

来源：

Chemosphere | 2024年 / 364卷

基金：

日本学术振兴会;

关键词：

Arsenate; Bioaccumulation; Freshwater phytoplankton; Phosphate; Single-cell ICP-MS;

D O I：

10.1016/j.chemosphere.2024.143129

中图分类号：

学科分类号：

摘要：

In this study, we investigated the interaction between arsenate (AsV) and phosphate (PO43−) in freshwater phytoplankton using single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS). This study aimed to elucidate the influence of varying PO43− concentrations on arsenic (As) uptake and distribution at the single-cell level, providing insights into intraspecies diversity. Two species of freshwater phytoplanktons, Scenedesmus acutus and Pediastrum duplex, were cultured under different concentrations of PO43− and AsV in a controlled laboratory environment. Scenedesmus acutus, a species with strong salt tolerance, and Pediastrum duplex, known for its weak salt tolerance, were selected based on their contrasting behaviors in previous studies. SC-ICP-MS revealed non-uniform uptake of As by individual phytoplankton cells, with distinct variations in response to PO43− availability. Arsenic uptake by both species declined with a high PO43− level after 7 days of exposure. However, after 14 days, As uptake increased in S. acutus with higher PO43− concentrations, but decreased in P. duplex. Moreover, our findings revealed differences in cell morphology and membrane integrity between the two species in response to AsV and various PO43− concentrations. S. acutus maintained cell integrity under all experimental culture conditions, whereas P. duplex experienced cell lysis at elevated AsV and PO43− concentrations. This study highlights the varying responses of freshwater phytoplankton to changes in AsV and PO43− levels and underscores the advantages of SC-ICP-MS over conventional ICP-MS in providing detailed, cellular level insights. These findings are crucial for understanding and managing As pollution in aquatic ecosystems. © 2024 Elsevier Ltd

引用

共 59 条

[1] Alam M.S., Fujisawa S., Zuka M., Zai Y., Mashio A.S., Rahman I.M.M., Wong K.H., Hasegawa H., Cellular uptake and biotransformation of arsenate by freshwater phytoplankton under salinity gradient revealed by single-cell ICP-MS and CT-HG-AAS, Environ. Chem., (2023)
[2] Amable L., Stephan C., Smith S., Merrifield R., An Introduction to Single Cell ICP-MS Analysis, (2017)
[3] Ashera C.J., Arsenic uptake by BarIey seedlings, Aust. J. Plant Physiol., 6, pp. 459-466, (1979)
[4] Awoyemi O.M., Subbiah S., Thompson K.N., Velazquez A., Peace A., Mayer G.D., Trophic-level interactive effects of phosphorus availability on the toxicities of cadmium, arsenic, and their binary mixture in media-exposed Scenedesmus acutus and media and dietary-exposed Daphnia pulex, Environ. Sci. Technol., 54, pp. 5651-5666, (2020)
[5] Azizur Rahman M., Hasegawa H., Arsenic in freshwater systems: influence of eutrophication on occurrence, distribution, speciation, and bioaccumulation, Appl. Geochem., 27, pp. 304-314, (2012)
[6] Azizur Rahman M., Hasegawa H., Peter Lim R., Bioaccumulation, biotransformation and trophic transfer of arsenic in the aquatic food chain, Environ. Res., 116, pp. 118-135, (2012)
[7] Bai X., Enhancing Algal Biomass and Biofuels Recovery from Open Culture Systems, (2015)
[8] Baker J., Wallschlager D., The role of phosphorus in the metabolism of arsenate by a freshwater green alga, Chlorella vulgaris, J. Environ. Sci. (China), 49, pp. 169-178, (2016)
[9] Baker M.D., Wong, Cwau Y.K., Mayfield C.I., Methylation of arsenic by freshwater green algae, Can. J. Fish. Aquat. Sci., 468, pp. 1254-1257, (1983)
[10] Byeon E., Kang H.M., Yoon C., Lee J.S., Toxicity mechanisms of arsenic compounds in aquatic organisms, Aquat. Toxicol., (2021)

← 1 2 3 4 5 6 →