Injectable hydrogel based on silk fibroin/carboxymethyl cellulose/agarose containing polydopamine functionalized graphene oxide with conductivity, hemostasis, antibacterial, and anti-oxidant properties for full-thickness burn healing

被引：22
作者：
Babaluei M. [1 ]
Mojarab Y. [1 ]
Mottaghitalab F. [2 ]
Farokhi M. [1 ]
机构：
[1] National Cell Bank of Iran, Pasteur Institute of Iran, Tehran
[2] Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
来源：
International Journal of Biological Macromolecules | 2023年 / 249卷
关键词：
Antibacterial; Conductivity; Full-thickness burn defect model; Injectable hydrogel; Wound dressing;
D O I：
10.1016/j.ijbiomac.2023.126051
中图分类号：
学科分类号：
摘要：
Overcoming bacterial infections and promoting wound healing are significant challenges in clinical practice and fundamental research. This study developed a series of enzymatic crosslinking injectable hydrogels based on silk fibroin (SF), carboxymethyl cellulose (CMC), and agarose, with the addition of polydopamine functionalized graphene oxide (GO@PDA) to endow the hydrogel with suitable conductivity and antimicrobial activity. The hydrogels exhibited suitable gelation time, stable mechanical and rheological properties, high water absorbency, and hemostatic properties. Biocompatibility was also confirmed through various assays. After loading the antibiotic vancomycin hydrochloride, the hydrogels showed sustained release and good antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The fast gelation time and desirable tissue-covering ability of the hydrogels allowed for a good hemostatic effect in a rat liver trauma model. In a rat full-thickness burn wound model, the hydrogels exhibited an excellent treatment effect, leading to significantly enhanced wound closure, collagen deposition, and granulation tissue formation, as well as neovascularization and anti-inflammatory effects. In conclusion, the antibacterial electroactive injectable hydrogel dressing, with its multifunctional properties, significantly promoted the in vivo wound healing process, making it an excellent candidate for full-thickness skin wound healing. © 2023 Elsevier B.V.
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