Simultaneous Determination of 13 Mycotoxins in Plant Solid Beverages by UPLC-MS/MS Combined with Modified QuEChERS

被引：0

作者：

Lu, Zhi ^{[1
]}

Li, Yaxian ^{[1
]}

Liu, Fengsong ^{[1
]}

Gao, Zhi ^{[1
]}

Li, Yajie ^{[1
]}

机构：

[1] Infinitus (China) Company Ltd., Guangzhou

来源：

Science and Technology of Food Industry | 2024年 / 45卷 / 21期

关键词：

high-throughput; mycotoxins; plant solid beverages; QuEChERS; ultra-performance liquid chromatography-tandem mass spectrometry;

D O I：

10.13386/j.issn1002-0306.2023120099

中图分类号：

学科分类号：

摘要：

This work aimed to develop a high-throughput analytical method based on QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of 13 mycotoxins in plant-solid beverages. The samples were homogenized and dissolved in water. QuEChERS method was used for pretreatment, ultrasonic extraction with 2% formic acid-acetonitrile solution, and purification with octadecylsilane bonded silica gel (C18). The extraction solution was then separated by a Kinetex F5 column, detected by a tandem mass spectrometry detector in multiple reaction monitoring mode (MRM), and quantified by matrix-matched standard calibration. The optimum conditions achieved limits of detections (LODs) in the range of 0.04~16.9 μg/kg, with correlation coefficient over 0.9916. The limit of quantifications (LOQs) were in the range 0.12~38.2 μg/kg. The recoveries in real plant-solid beverages ranged from 82.2% to 103.3%, with relative standard deviations (RSDs) below 9.0%. Moreover, the proposed method was applied to analyze mycotoxins in 18 plant-solid beverage samples, a positive detection rate of 4/18 was obtained, and the detected analytes included deoxynivalenol and fumonisin B3. This proposed method has the advantages of simple operation, low cost, high sensitivity and accuracy, which can be used for the high-throughput screening of multiple mycotoxins in plant-solid beverages. © The Author(s) 2024.

引用

页码：275 / 282

页数：7

共 30 条

[1]

OUYANG X H., Safety problems and supervision suggestions of solid beverage industry[J], China Food Safety Magazine, 16, 32, (2022)

[2]

ALSHANNAQ A, YU J H., Occurrence, toxicity, and analysis of major mycotoxins in food[J], International Journal of Environmental Research and Public Health, 14, 6, (2017)

[3]

KESKIN E, EYUPOGLU O E., Determination of mycotoxins by HPLC, LC-MS/MS and health risk assessment of the mycotoxins in bee products of Turkey[J], Food Chemistry, 400, (2023)

[4]

DO T H, TRAN S C, LE C D, Et al., Dietary exposure and health risk characterization of aflatoxin B1, ochratoxin A, fumonisin B1, and zearalenone in food from different provinces in Northern Vietnam[J], Food Control, 112, (2020)

[5]

XU Y D, YUAN X, WANG H M, Et al., Research progress in detection of mycotoxins by chromatography[J], Science and Technology of Cereals, Oils and Foods, 26, 6, (2018)

[6]

YAN J X, HU W J, YOU K H, Et al., Biosynthetic mycotoxin conjugate mimetics-mediated green strategy for multiplex mycotoxin immunochromatographic assay[J], Journal of Agricultural and Food Chemistry, 68, 7, pp. 2193-2199, (2020)

[7]

WANG H B, LI P W, ZHANG Q, Et al., A review on progress of preparation of antibodies against mycotoxins in foodstuff and oilseeds[J], Chinese Journal of Oil Crop Sciences, 34, 3, (2012)

[8]

WU D F, WANG L, DAI P Z, Et al., Simultaneous detection of six mycotoxins in tea by HPLC with purification on immunoaffinity column[J], Analytical Instrumentation, 54, 1, (2023)

[9]

AREO O M, ABAFE O A, GBASHI A S, Et al., Detection of multi-mycotoxins in rooibos and other consumed teas in South Africa by a modified QuEChERS method and ultra-high performance liquid chromatography tandem mass spectrometry[J], Food Control, 143, (2023)

[10]

ZHOU H Y, YAN Z, YU S, Et al., Development of a novel UPLC-MS/MS Method for the simultaneous determination of 16 mycotoxins in different tea categories[J], Toxins, 14, 3, (2022)

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