Tibetan Sheep Meat Energy Metabolism, Apoptosis and Tenderization during Postmortem Aging after H2O2 Treatment

被引:0
|
作者
Shi X. [1 ]
Yue J. [1 ]
Zhang P. [1 ]
Tian Z. [1 ]
Chen C. [1 ]
Ma X. [1 ]
机构
[1] College of Food Science and Engineering, Gansu Agricultural University, Lanzhou
来源
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | 2021年 / 52卷 / 03期
关键词
Apoptosis; Energy metabolism; Hydrogen peroxide; Tenderization; Tibetan sheep meat;
D O I
10.6041/j.issn.1000-1298.2021.03.040
中图分类号
学科分类号
摘要
In order to clarify the effect of H2O2 treatment on energy metabolism, apoptosis and tenderization in the postmortem aging of Tibetan sheep meat. The Longissimus dorsi (LD) of Tibetan sheep was selected as the experimental material, Tibetan sheep meat was treated with distilled water, 50 mmol/L and 200 mmol/L reactive oxygen activator hydrogen peroxide, and then aged at 4℃ for 0 d, 0.5 d, 1 d, 3 d, 5 d and 7 d. The changes in ROS relative content, ATP content, Ca2+-ATPase and Na+/K+-ATPase activity, apoptosis rate, myofibril fragmentation index (MFI) and muscle fiber diameter during postmortem aging were tested. The results showed that after aging of 7 d, the relative content of ROS in the 50 mmol/L H2O2 treatment group was 39.19% significantly higher than that of the control group (P< 0.05), the ATP content, Ca2+-ATPase and Na+/K+-ATPase activity and muscle fiber diameter of 50 mmol/L and 200 mmol/L H2O2 treatments were 25.73% and 25.77%, 38.39% and 36.70%, 27.30% and 29.46%, and 13.68% and 13.94% significantly lower than that of the control group (P< 0.05), the apoptosis rate and MFI in the two treatment groups were 63.07% and 50.42%, and 5.74% and 5.19% significantly higher than that of the control group (P< 0.05). To sum up, the H2O2 treatmented Tibetan sheep meat could promote ROS production, change energy metabolism of the Tibetan sheep meat, improve the activity of apoptosis, and promote the meat tenderization. It provided a theoretical basis for improving the quality of Tibetan sheep meat. © 2021, Chinese Society of Agricultural Machinery. All right reserved.
引用
收藏
页码:357 / 363
页数:6
相关论文
共 41 条
  • [1] MAO Xuerong, Analysis mutton quality of Oura-type of Tibetan sheep, Chinese Qinghai Journal of Animal and Veterinary Sciences, 3, pp. 3-4, (2005)
  • [2] LI Mengqi, Quality analysis and the effects of frozen storage on lipid and protein oxidation of Oula Tibetan sheep meat, (2018)
  • [3] CHEN Xuemin, ZHU Guoyin, LUO Hailing, Et al., Quantitative analysis of Oula lamb meat volatile compounds based on fignerprint, Transactions of the Chinese Society for Agricultural Machinery, 51, 5, pp. 349-355, (2020)
  • [4] KEMPP C M, SENSKY P L, BARDSLEY R G, Et al., Tenderness-an enzymatic view, Meat Science, 84, 2, pp. 248-256, (2010)
  • [5] XU Qiongqiong, HAN Bingshe, LUO Juntao, Et al., Effects of low temperature stress on expression of ROS and MAPK-related proteins in zebrafish ZF4 cells, Journal of Fishery Sciences of China, 23, 4, pp. 771-776, (2016)
  • [6] XUE Mei, Influence of protein oxidation on myofibrillar proteins degradation and meat eating quality from bovine during postmortem ageing, (2012)
  • [7] GABRIEL B, SUREAU F, CASSELYN M, Et al., Retroactive pathway involving mitochondria in electroloaded cytochrome c-induced apoptosis. Protective properties of Bcl-2 and Bcl-XL, Experimental Cell Research, 289, 2, pp. 195-210, (2003)
  • [8] LIU Yanying, FENG Qingkun, YANG Haiping, Mitochondrial homeostasis imbalance and myopenia, Journal of Zhaoqing University, 41, 2, pp. 81-85, (2020)
  • [9] ZHANG Xiaotao, PAN Daodong, CAO Jinxuan, Et al., Changes in the major caspases involved in cytoskeletal degradation of goose muscle during prolonged aging, Food Research International, 51, 2, pp. 603-610, (2013)
  • [10] XU Jingjing, DAI Xiaomin, LIU Hailiang, Et al., A novel 7-azaisoindigo derivative-induced cancer cell apoptosis and mitochondrial dysfunction mediated by oxidative stress, Journal of Applied Toxicology, 31, 2, pp. 164-172, (2015)